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Pharma & Biotech

P1 Primary Cell 4D-NucleofectorTM X Kit S

Catalog #: V4XP-1032
For efficient transfection of specific primary cells, e.g. human ADSCs or MSCs, in the 4D-NucleofectorTM X Unit (20 µL format).
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Product Overview

The P1 Primary Cell 4D-NucleofectorTM X Kit S is one of our five 4D-NucleofectorTM Kits suited for transfecting primary cells in the 20 µL 16-well NucleocuvetteTM Strip format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:

  • ADSC undiff. (human)
  • MSC (human)
  • Preadipocytes (human)
  • PrEC (human)

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

Benefits

  • High transfection efficiencies for primary cells
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System

Applications

  • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
  • Medium throughput applications
  • Optimization of transfection conditions e.g. substrate titrations

Content & Storage

Content

2 x 0.675 mL P1 Primary Cell NucleofectorTM Solution
2 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well NucleocuvetteTM Strips (20 μl)

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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Browse our Cell and Transfection, Citation, FAQ and Culture Media Technical Databases.

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