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SG Cell Line 4D-Nucleofector™ X Kit L

SG Cell Line 4D-Nucleofector™ X Kit L

Catalog #: V4XC-3024
For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-NucleofectorTM X Unit (100 µL format).
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 550.00 CHF
SA

Product Overview

The SG Cell Line 4D-NucleofectorTM X Kit L is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL NucleocuvetteTM Vessel format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 12 or 24 reactions.

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Benefits

  • High transfection efficiencies in difficult-to-transfect cell lines
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System

Applications

  • Transfection of cell numbers in the range of 2 x 105 to 2 x 107 cells
  • Low throughput applications

Storage and Content

2.25 mL SG Cell Line NucleofectorTM Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL NucleocuvetteTM Vessel
24 x Single use pipettes

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Raji Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of Raji cells with the SG Cell Line 4D-Nucleofector™ X Kit
  • NIH/3T3 Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of NIH/3T3 cells with the SG Cell Line 4D-Nucleofector™ X Kit
  • THP-1 Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of THP-1 cells with the SG Cell Line 4D-Nucleofector™ X Kit
  • Ramos Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of Ramos cells with the SG Cell Line 4D-Nucleofector™ X Kit
  • CHO-S [Suspension] Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of CHO-S cells (Invitrogen) with the SG Cell Line 4D-Nucleofector™ X Kit
  • BA/F3 Transfection Protocol - 4D-Nucleofector™ Device

    Instructions of use for transfection of BA/F3 cells (DSMZ) with the SG Cell Line 4D-Nucleofector™ X Kit
  • H4 Transfection Protocol - 4D-Nucleofector™ X-Unit

    Instructions of use for transfection of H4 cells with the SG Cell Line 4D-Nucleofector™ X Kit
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