SF Cell Line 4D-Nucleofector™ X Kit L
What You May Also Need
Product Overview
The SF Cell Line 4D-NucleofectorTM X Kit L is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL NucleocuvetteTM Vessel format when working with the 4D-NucleofectorTM X Unit.
This kit has been determined to be optimal for transfecting following cell types:
- 293-F (FreeStyle), 32D
- A-20, A2058, A375, A431, A549, AGS, ARPE-19
- BetaTC/6, BxPC-3
- C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1
- EL4
- FDC-P1
- H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C
- IMR-32
- J774A.1
- K-562
- KG-1
- L-428, LNCaP
- MDA-MB-453, MDBK, Molt-4, MV-4-11
- Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0
- P19, P815, PC-12, PC-3
- Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226
- S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480
- T2, T84, TF-1
- U266B1, U-937
- Vero
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 12 or 24 reactions.
Benefits
- High transfection efficiencies in difficult-to-transfect cell lines
- Cells maintain functionality post transfection
- Conditions are transferable between 4D-NucleofectorTM System (including the , 96-well ShuttleTM System and 384-well NucleofectorTM System
Applications
- Transfection of cell numbers in the range of 2 x 105 to 2 x 107 cells
- Low throughput applications
Content & Storage
Content
2.25 mL SF Cell Line NucleofectorTM Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL NucleocuvetteTM Vessel
24 x Single use pipettes
