Product Overview
EBM®-2 is an endothelial basal medium sold separately. This can be used with 1 x kit of CC-4176 (Large-vessel endothelial cell growth medium SingleQuots® Supplements and Growth Factors) or 1 x CC-4147 (Microvascular endothelial cell growth medium SingleQuots® Supplements and Growth Factors).
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow CloneticsTM Primary Cells.
- EGM®-2 BulletKit® (CC-3162) – Optimized for certain HUVECs, Aortic and Pulmonary artery endothelial cells. Contains 2% FBS and VEGF for rapid proliferation. Does not contain BBE.
- EGM®-2 MV BulletKit® (CC-3202) – Optimized for Coronary and Iliac artery endothelial cells and human microvascular endothelial cells, contains 5% FBS and VEGF for rapid proliferation. Does not contain BBE.
Lonza guarantees the performance of Clonetics®/Poietics® Cells only if appropriate Clonetics®/Poietics® Media and Reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees.
If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.
Request More InfoStorage and Content
1 x Bottle EBM®-2 Basal Medium, 500 mL
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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TechSheet – Endothelial Cell System Medium Products
Technical information sheet for Clonetics® Endothelial Cell Medium Products -
NPCs Hepatic Non-Parenchymal Cells
These additional liver cell types provide a complete building set supporting complex cell culture modeling. Applications include developing more physiologically relevant in vitro models for complex liver diseases and toxicity testing of new drugs and chemicals. -
Instructions & Technical Info - Endothelial Cell Systems
Technical Information and Instructions for Proper Use of Endothelial Cells -
Primary Human Umbilical Vein Endothelial Cells
Tech note demonstrating the successful development of tube formation assay using primary HUVECs that can be used to visualize angiogenesis in real-time. -
A Quantitative, High-throughput, Real-time Angiogenic Tube Formation Assay
Angiogenic Assay - Human Umbilical Vein Endothelial Cells and Human Dermal Fibroblasts in Co-culture
Educational Material
Brochures, White Papers etc.
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Poster - AACR 2017
Simple and easy monitoring of tube formation and migration assays with the CytoSMART™ Live Cell Imaging System -
White Paper
Simple and easy monitoring of three different cancer-relevant assays – Tube formation assay, 2D cell migration assay and 3D cell invasion assay -
Assessment of the Anti-angiogenic Effect of VEGFR2 siRNA in HUVEC
Scientific poster for AACR 2015 -
First siRNA Library Screening in Hard-to-Transfect HUVEC and Jurkat Cells
High throughput transfection of siRNA libraries has become a valuable tool in target identifi cation and validation. However, such screenings have so far been constrained to mostly easy-totransfect adherent cell lines. Lonza’s 96-well Shuttle® System, based on the well-established Amaxa® Nucleofector® Technology, extends these approaches to primary and diffi cult-to-transfect cells. -
Tube Formation Assay with Primary HUVECs
In this study, we have optimized this assay using Lonza's Primary HUVECs and EGM®-2 Media -
A Quantitative, High-throughput, Real-time Angiogenic Tube Formation Assay
Angiogenic Assay - Human Umbilical Vein Endothelial Cells and Human Dermal Fibroblasts in Co-culture
