384-well Nucleofector® System
384-well Nucleofector® System
Product Overview
The 384-well Nucleofector® System is an independent platform for high-throughput Nucleofection in a 384-well format. With an extremely fast plate processing time of 1 minute per plate, it is perfectly suited for screening applications with maximum reproducibility.
The 384-well Nucleofector® System consists of a Power Supply Unit generating the high voltage pulses, a Plate Handler Unit and an intuitive PC-based Operation Software.
The 384-well Nucleofector® Kits use existing 4D-Nucleofector® 96-well Unit Protocols with conductive polymer 384-well Nucleocuvette® Plates.
There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Technical Specifications
Dimensions (w × d × h):
- 384-well Nucleofector® Plate Handler: 40 cm × 42 cm × 15 cm (15.7 × 16.5 × 5.9 in)
- 384-Nucleofector® Power Supply: 13.5 cm × 50 cm × 45 cm (5.3 × 19.6 × 17.7 in)
Weight:
- 384-well Nucleofector® Plate Handler: 10 kg (22.04 lb)
- 384-well Nucleofector® Power Supply: 14 kg (30.86 lb)
Laptop operating system: Windows 7 or 10
Request More InfoBenefits
- Fast - Processes a 384-well plate in one minute, carousel capable of handling two plates
- High performance combined with minimum material consumption - Nucleofection of low cell numbers down to 2x104 cells
- Easy-to-use - Uses existing 4D-Nucleofector® 96-well Unit Protocols
- Automation compatible - Seamless integration into automated liquid handling environments
Applications
- High-throughput Nucleofection of low cell numbers down to 2 × 104 cells
- Screening of arrayed cDNA, RNAi or CRISPR libraries
Storage and Content
1 HT Nucleofector® Plate Handler
1 HT Nucleofector® Power Supply
1 Laptop with HT Nucleofector® Software
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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HT Nucleofector® Hardware Manual
Lonza_ManualsProductInstructions_HT_Nufe_hardware_manual_sec.pdf -
Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide
The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA. -
Important Vector Factors for Gene Expression – Technical Reference Guide
In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results. -
Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide
This document will provide you with guidelines on how to prepare quality DNA for transfections.
Educational Material
Brochures, White Papers etc.
Videos
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Designing an RNAi Experiment using Nucleofection® – Technical Reference Guide
The success of an RNAi experiment can be influenced by several parameters. This guide describes the critical steps for setting up an RNAi experiment with siRNA oligos. Partially the guidelines also apply for shRNA. -
Important Vector Factors for Gene Expression – Technical Reference Guide
In this guideline, we show the most important aspects to be considered when choosing an expression vector and their impact on your experimental results. -
Essentials for Preparing a Transfection Experiment - Plasmid DNA - Technical Reference Guide
This document will provide you with guidelines on how to prepare quality DNA for transfections. -
384-well Nucleofector System
384-well Nucleofector System - Designed for High-throughput Transfection
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How it Works - HT Nucleofector® System (Video Tutorial)
Workflow overview for using the HT Nucleofector™ System
