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P2 Primary Cell 384-well Nucleofector® Kit
Catalog #: V5SP-2010
For high-throughput transfection of primary cells, e.g. mouse macrophages or human fibroblasts, when working with the 384-well HT Nucleofector® System.
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled, in EGM™-2, Prescreened for Angiogenesis
Catalog #: C2519AS
Cryopreserved ampule of Human Umbilical Vein Endothelial Cells (HUVEC) from pooled donors, cultured in EGMTM-2, pre-screened for Angiogenesis and containing ≥ 500,000 cells
AD2 4D-Nucleofector® Y Kit
Catalog #: V4YP-2A24
For adherent electroporation of primary cells, e.g. fibroblasts, when working with the 4D-Nucleofector® Y Unit.
RtEGMTM Retinal Epithelial Cell Growth Medium SingleQuots® Supplements and Growth Factors
Catalog #: 00195407
Retinal Epithelial Growth Medium (RtEGM) SingleQuots® Supplements and Growth Factors
Primary Cell Optimization 4D-Nucleofector® X Kit
Catalog #: V4XP-9096
For rapid determination of Nucleofection conditions for primary cell types lacking an Optimized Protocol for the 4D-Nucleofector® X Unit.
P2 Primary Cell 384-well Nucleofector® Kit
Catalog #: V5SP-2002
For high-throughput transfection of primary cells, e.g. mouse macrophages or human fibroblasts, when working with the 384-well HT Nucleofector® System.
D-HLF-COPD – Diseased Human Lung Fibroblast Cell – COPD
Catalog #: 00195277
DHLF-COPD, Lung Fibroblasts, COPD
Pro293TMa Serum-free Medium – Chemically Defined for 293 Adherent Cells
Catalog #: BEBP12-764Q
Pro293TMa Serum-free Medium, chemically defined for adherent cells, without L-glutamine or phenol red with 0.1% Pluronic® F-68, 1 L
hMSC Human Mesenchymal Stem Cell Adipogenic Maintenance Medium SingleQuots® Supplements and Growth Factors
Catalog #: PT-4122
hMSC Adipogenic Maintenance SingleQuots® Kit
HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor, in EGM™-2, Prescreened for Angiogenesis
Catalog #: C2517AS
HUVEC, single donor, in EGMTM-2, Pre-screened for angiogenesis
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive.
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Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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