For efficient transfection of human stem cells in the Nucleofector® I/II/2b Device
Culture system containing SmBM® Basal Medium (CC-3181) and SmGM®-2 SingleQuots® supplements (CC-4149) required for growth of Smooth Muscle Cells
Culture system containing SmBM® Basal Medium (CC-3181) and SmGM®-2 SingleQuots® supplements (CC-4149) required for growth of Smooth Muscle Cells (Pack of 6)
Cryopreserved ampule of Human Subcutaneous Preadipocytes containing ≥1 million cells
Cryopreserved Normal Human Bronchial Epithelial (NHBE) cells, screened and guaranteed for differentiation, in B-ALITM (Bronchial Air Liquid Interface) cell culture.
Cryopreserved ampule of Normal Human Bronchial Epithelial (NHBE) cells with Retinoic Acid containing ≥500,000 cells
Serum-free growth medium required for growth of human lymphocytes and dendritic cells, 500 mL
For adherent electroporation of primary cells, e.g. neuronal or endothelial cells, when working with the 4D-Nucleofector® Y Unit.
D-SAEC-COPD, Small Airway Epithelial Cells from donors with COPD
Cryopreserved ampule of Neonatal Normal Human Epidermal Keratinocytes (NHEK-Neo), Single Donor, containing ≥ 500,000 cells
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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References - X-Vivo™Overview of relevant references
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
Terms and ConditionsCD34+ cells BOGO
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Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit