text.skipToContent text.skipToNavigation
Need help? Please contact us

P4 Primary Cell 4D-Nucleofector™ X Kit L

P4 Primary Cell 4D-Nucleofector™ X Kit L

Catalog #: V4XP-4024
For efficient transfection of specific primary cells, e.g. mouse B-cells or mouse immature DCs, in the 4D-NucleofectorTM X Unit (100 µL format).
Compare

Product Overview

The P4 Primary Cell 4D-NucleofectorTM X Kit L is one of our five 4D-NucleofectorTM Kits suited for transfecting primary cells in the 100 µL NucleocuvetteTM Vessel format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:

  • B cell (mouse)
  • DC (mouse), immat.
  • MEF (mouse)

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 12 or 24 reactions.

Request More Info

Benefits

  • High transfection efficiencies for primary cells
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System

Applications

  • Transfection of cell numbers in the range of 2 x 105 to 2 x 107 cells
  • Low throughput applications

Storage and Content

2.25 mL P4 Primary Cell NucleofectorTM Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
24 x 100 µL NucleocuvetteTM Vessel
24 x Single use pipettes

Return to Top

SDS, CoA, and Instructions

Safety Data Sheets (SDS)

Choose a language to view the SDS.

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Transfection of Human Induced Pluripotent Stem Cells using Nucleofector® Technology

    Technical Reference Guide providing tips for transfecting iPSCs using the Nucleofector® Technology
Return to Top

Educational Material

Brochures, White Papers etc.

  • Transfection of Human Induced Pluripotent Stem Cells using Nucleofector® Technology

    Technical Reference Guide providing tips for transfecting iPSCs using the Nucleofector® Technology
Return to Top