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P2 Primary Cell 4D-Nucleofector™ X Kit SCatalog #: V4XP-2032For efficient transfection of specific primary cells, e.g. mouse macrophages and NHDF, in the 4D-NucleofectorTM X Unit (20 µL format).Sales unit: kit
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RPMI 1640 50L powderCatalog #: BE15-398FRPMI 1640 without Phenol Red, NaHCO3 and L-Glutamine, 50L powderSales unit: each
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EBMTM-2 Endothelial Cell Growth Basal Medium-2, 1 LCatalog #: 00190860Basal media required for growth of endothelial cells, 1 L (supplements and growth factors sold separately).Sales unit: Bottle
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XtremeFeed™– Chemically Defined and Protein-free Basal Powder 200 LCatalog #: VPW-098QXtremeFeed™ CD , Protein free, Basal Powder 200LSales unit: each
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ProCHO® 5 Protein-free CHO Medium Powder kit, 500 LCatalog #: BE15-766L
ProCHO® 5 Protein-free CHO Medium Powder kit with HEPES and 0.1% Pluronic® F-68, without L-Glutamine, phenol red, hypoxanthine or thymidine, 500 L (NAO)
Sales unit: kit -
FlashGel™ DNA CassettesCatalog #: 57023
FlashGelTM DNA Cassettes, 1.2% 12+1 well single-tier, 9 pack
Separation: 50 bp - 4 kb (up to 10 kb with lower voltage/longer run)
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FlashGel™ DNA CassettesCatalog #: 57031
FlashGelTM DNA Cassettes, 2.2% 12+1 well single-tier, 9 pack
Ideal for testing 12 samples with small DNA fragments (e.g. PCR products)
Separation: 10 bp - 1 kb
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Cell Line Optimization Nucleofector™ Kit for Nucleofector™ DeviceCatalog #: VCO-1001NFor determination of the optimal transfection conditions for any difficult-to-transfect cell line that lacks an Optimized Protocol for the NucleofectorTM I/II/2b DeviceSales unit: items
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Mouse Embryonic Fibroblasts (MEF)Catalog #: M-FB-481Cryopreserved ampule of Mouse Embryonic Fibroblasts containing ≥2 million cellsSales unit: each
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Irradiated Mouse Embryonic Fibroblasts (iMEF)Catalog #: M-IFB-482
Cryopreserved ampule of Irradiated Mouse Embryonic Fibroblasts containing ≥2 million cells
Sales unit: each
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TechSheet - Umbilical Vein Endothelial Cell SystemsTechnical information sheet for CloneticsTM HUVEC cells
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Instructions for Use - HUVEC Culturing and Angiogenesis Inhibition Assay using Sartorius Incucyte®Protocol for Angiogenesis assay using HUVEC cells
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Expanded HUVECs for High-throughput ScreeningHUVECs are a good primary endothelial cell type for many in vitro studies and also for several high-throughput screening applications. Consequently, Lonza offers, HUVEC-XL. These cells are expanded to passage-3 from pooled P1-HUVECs and packaged at 10 million cells per ampoule.
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Tube Formation Assay with HUVEC
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HUVEC-Fibroblast Co-Culture - Angiogenesis
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Tube Formation Assay with Primary HUVECsIn this study, we have optimized this assay using Lonza's Primary HUVECs and EGM™-2 Media
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HUVEC-Fibroblast Co-Culture – Angiogenesis Inhibition
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Instructions - HUVEC-XL™ Pooled Cell SystemInstructions for culturing Clonetics™ HIVEC-XL™Pooled Cells
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Automated Cell Signaling Assays Using Primary HUVECsLonza's Primary HUVECs are an excellent cell type to measure the effects of pharmacologically-important compounds on vial signaling pathways, and they provide increased data relevancy compared to cell lines.
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Lonza Extends Industry-Standard HUVEC Product LineLonza, the market leader in primary cells, has expanded its human primary cell portfolio with an improved version of Human Umbilical Vein Endothelial Cells (HUVEC) cultured in the absence of additive vascular endothelial growth factor (VEGF).
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