For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-NucleofectorTM X Unit (20 µL format).
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Cryopreserved ampule of Rat Neonatal DRG Neurons containing ≥ 200,000 cells
Cryopreserved ampule of Mouse Brain CD1 Hippocampus Neurons containing ≥ 1,000,000 cells
Cryopreserved ampule of Human Renal Proximal Tubule Epithelial Cells (RPTEC) containing ≥ 500,000 cells
Do you need a modified version of this cell product, such as different vialing sizes, or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research
The PyroWaveTM XM Fluorescence Reader brings a new generation in fluorescence technology to users of the PyroGeneTM Recombinant Factor C Endotoxin Detection Assay. This fluorescence microplate reader is optimized for Lonza’s PyroGeneTM Assay, bringing new and improved technology to the laboratory.
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Transfection Protocol for NHBE - 4D-Nucleofector™ DeviceInstructions of use for transfection of normal human bronchial epithelial cells (Lonza) with the P3 Primary Cell 4D-Nucleofector™ X Kit
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B-ALI Immune-Airway Co-Culture Model: Normal Human Bronchial Epithelial cells and Peripheral Blood Mononuclear Cells - Instructions for useProtocol to culture B-ALI NHBE cells with PBMCs
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Transfection Protocol for NHBE - 96-well Shuttle™ DeviceInstructions of use for transfection of normal human bronchial epithelial cells (Lonza) with the P3 Primary Cell 96-well Nucleofector™ Kit
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Viable Cells - Proliferating Clonetics™ Cells Chart
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Transfection Protocol for NHBE - Nucleofector™ I/II/2b Device SeriesInstructions of use for transfection of normal human bronchial epithelial cells (Lonza) with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
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Primary Cells and Media Reference GuideA comprehensive collection of Lonza primary cell and media products supporting many research areas.
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Gene Expression Differences in Airway Epithelial Cells from Asthma or COPD DonorsAccess to diseased primary cells from patients diagnosed with COPD or asthma provides a convenient, biologically relevant model to assess genetic and in some cases phenotypic changes from normal, healthy donor tissues. In this study, we grew human bronchial epithelial (HBE) cells from normal, COPD-, and asthma-diagnosed human donor lung tissues in an air-liquid interface (ALI) model to assess potential phenotypic changes in vitro as well as provide a differentiated layer of cells to harvest for gene expression studies using the StellARray™ Gene Expression System.
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TechSheet - Bronchial, Tracheal Epithelial Cell SystemsTechnical information sheet for Clonetics™ Bronchial and Tracheal cells
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Comparison of Normal and Asthmatic Bronchial Epithelial Cells and Smooth Muscle Cells in Monolayer and RAFT™ 3D Cell Culture SystemUnderstanding the differences between normal and diseased lung cells in 2D vs 3D culture.
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Instructions - Airway Epithelial Cell SystemsInstructions for Proper Use and Culture of Airway Epithelial Cells