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SF Cell Line 4D-Nucleofector™ X Kit S

Catalog #: V4XC-2032

For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-NucleofectorTM X Unit (20 µL format).


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 507.00 CHF

Product Overview

The SF Cell Line 4D-NucleofectorTM X Kit S is one of our three 4D-NucleofectorTM Kits that is suited for transfecting difficult-to-transfect cell lines in the 20 µL 16-well NucleocuvetteTM Strip format when working with the 4D-NucleofectorTM X Unit.

This kit has been determined to be optimal for transfecting following cell types:


The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.


  • High transfection efficiencies in difficult-to-transfect cell lines
  • Cells maintain functionality post transfection
  • Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System
  • Transferability to 4D-NucleofectorTM LV Unit


  • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
  • Medium throughput applications
  • Optimization of transfection conditions e.g. substrate titrations

Content & Storage


1 x 0.675 mL SF Cell Line Nucleofector®; Solution
1 x 0.15 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
2 x 16-well Nucleocuvette® Strips (20 μL)


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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