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Human CD34+ Cell Nucleofector™ Kit

Catalog #: VPA-1003
For efficient transfection of primary human CD34+ cells in the NucleofectorTM I/II/2b Device

Product Overview

The NucleofectorTM 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This NucleofectorTM Kit is the optimal kit for efficient transfectiion of primary human hematopoietic stem cells (CD34+) from cord blood or bone marrow.

The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.



  • High transfection efficiencies for primary cells
  • Cells maintain functionality post transfection


  • Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells 
  • Low-throughput transfection in single cuvette format

Content & Storage


2.25 mL NucleofectorTM Solution for Human CD34+ Cells
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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Knowledge Center

Need technical data?

Browse our Cell and Transfection, Citation, FAQ and Culture Media Technical Databases.