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4D-Nucleofector® LV Unit – Large volume transfection for ex vivo cell and gene therapy

4D-Nucleofector® LV Unit for Cell and Gene Therapy Applications – Benefits and How it Works

From this video learn how it works and its benefits for cell and gene therapy manufacturing.

Benefit from:

  • Closed system: Sterile transfection of up to 109 cells
  • Real scalability: Optimization in small scale
  • Established protocols: Benefit from 700+ optimized cell types
  • Simple handling: Minimal training needs
  • 4D-Nucleofector® LogWare: Optional operation via 21CFR part11 compliant software
  • Fit for purpose: consumables for research use and GMP manufacturing*

Applications:

  • Efficient transfection of human primary blood cells, e.g. T cells or NK cells
  • CRISPR genome editing using plasmid, mRNA or RNPs
  • CAR-T cell generation using transposon-based systems or mRNA

Optimal Transfection and Culture Conditions for Large-Scale Electroporation of T Cells

Download this poster and find out how our scientists have optimized transfection and culture protocols for primary human T cells by scaling the process from about 1 million cells in 20 μL to 1 billion in 20 mL using the 4D-Nucleofector® Platform.

Formats available

Large-scale transfection

1  of 
1  of 

Selected publications – Pre-clinical research

Selected publications – Clinical studies

Selected publications – Clinical studies (Other units)

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* TheraPEAK® Nucleofector® Products are produced according to applicable GMP raw material standards and are intended to support GMP manufacturing. TheraPEAK® Nucleofector® Solutions are manufactured at a GMP certified site. TheraPEAK® Nucleofector® Cartridges and Reservoirs are produced at manufacturing sites with an ISO 13485 certified quality management system. TheraPEAK® Nucleofector® Products are not intended for in vivo and/or diagnostic purposes. All other Nucleofector® Products are for research use only.