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Pharma & Biotech

Human Mesenchymal Stem Cell (hMSC) Chondrogenic Differentiation Medium BulletKitTM

Catalog #: PT-3003

Culture system containing hMSC Chondrogenic Basal Medium (PT-3925) and hMSC Chondrogenic SingleQuotsTM Kit Supplement (PT-4121) required for hMSC chondrogenic differentiation

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Product Overview

Stem cells possess the unique ability to differentiate into multiple lineages of cells including chondrocytes. Lonza’s hMSC Chondrogenic Differentiation BulletKitTM Medium is a serum containing medium designed to induce chondrogenic differentiation, with the addition of TGF-β3, of human bone marrow derived mesenchymal stem cells into mature, functionally active chondrocytes in as few as 14 days. Differentiation can then be validated by a variety of methods including staining with alcian blue or safranin.

Partial List of Cells Differentiated with hMSC Chondrogenic Differentiation BulletKitTM Medium*:

  • Human bone marrow derived stem cells
  • Human umbilical cord blood derived stem cells
  • Human umbilical cord matrix derived stem cells
  • C57BL/6 mice bone marrow derived stem cells
  • human adipose derived stem cells
  • Human hamstring tendon derived stem cells

The hMSC Chondrogenic Differentiation BulletKitTM Medium, with the addition of TGF-β3 (sold separately) provides a differentiation medium guaranteed to induce chondrogenic differentiation of bone marrow derived hMSCs into mature, functionally active chondrocytes; Each bottle of the hMSC Chondrogenic Differentiation BulletKitTM Medium contains enough differentiation medium to fully differentiate at least 2 million bone marrow derived hMSC [approximately 4 complete 24-well plates] into mature, functionally active chondrocytes.

The hMSC Chondrogenic Differentiation Medium is offered as a BulletKitTM Medium (catalog no. PT-3003) which includes both the basal media and the necessary supplements (with the addition of TGF-β3 [sold separately, catalog no. PT-4124]) for chondrogenic differentiation of bone marrow derived hMSCs. The basal medium (Differentiation Basal Medium - Chondrogenic; catalog no. PT-3925) and the necessary supplements (HMSC SQ Kit - Chondro; catalog no. PT-4121 and TGF Beta 3 for HMSC Chondro diff; catalog no. PT-4124) can also be purchased individually.

Lonza guarantees the performance of cells only if appropriate media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

*List of cells generated from customer data. Lonza only routinely uses this medium with Lonza’s human bone marrow derived mesenchymal stem cells and can therefore only guarantee the successful use of this media with these cells.

Content & Storage

Content

1 x hMSC Chondrogenic Basal Medium (PT-3925)

1 x hMSC Chondrogenic SingleQuot Kit (PT-4121)

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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