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Pharma & Biotech

MycoAlertTM Mycoplasma Detection Kit (25 Tests)

Catalog #: LT07-218
Simple and fast detection of mycoplasma contamination in cell cultures using the first generation MycoAlertTM Mycoplasma Detection Kit (25 Tests).

Product Overview

The MycoAlertTM Mycoplasma Detection Kit (25 Tests) is a selective biochemical test that exploits the activity of mycoplasma enzymes which are found in all six of the main mycoplasma cell culture contaminants and the vast majority of 180 mycoplasma species, but are not present in eukaryotic cells.
Viable mycoplasma in a test sample (cell supernatant) are lysed and the enzymes react with the MycoAlertTM Substrate, catalyzing the conversion of ADP to ATP. The ATP is then transferred into a light signal via the luciferase enzyme in the MycoAlertTM Reagent. By measuring the level of ATP in a sample both before (read A) and after the addition of the MycoAlertTM Substrate (read B), a ratio can be obtained which is indicative of the presence or absence of mycoplasma.


  • Convenient and fast
  • Simply add two reagents to the culture supernatant
  • Perform two luminescence readings
  • Results in less than 20 min
  • Easy interpretation of results 
  • Detects all common mollicute contaminations (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
  • Negligible interference with media components

Content & Storage


5 x 600 µL MycoAlertTM Reagent (lyophilized)
5 x 600 µL MycoAlertTM Substrate (lyophilized)
1 x 10 mL MycoAlertTM Assay Buffer


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

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Know Your Enemy – Mycoplasma Contamination in Cell Culture


Watch this webinar on mycoplasma testing and learn about potential impacts of a contamination on cell cultures and how you can easily uncover and remove it. 

Get More Info

Interested in Using MycoAlertTM Assays But No Luminometer at Hand?

Check out the LucettaTM 2 Luminometer for performing MycoAlertTM Assays right next to your cell culture hood. Mycoplasma detection couldn't be more convenient.

Request a Trial

Interested in Testing the MycoAlertTM PLUS Assay?

If you are interested in testing the MycoAlertTM Plus Assay, request a trial kit at a 50% discount. The trial kit includes the MycoAlertTM PLUS Assay (10 tests) and the MycoAlertTM Assay Control Set for positive control.