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Pharma & Biotech

4D-NucleofectorTM LV Unit

Catalog #: AAF-1002L

For closed, scalable large-scale transfection of up to 1x109 cells


Product Overview

Large-Scale Transfection Using the 4D-NucleofectorTM LV Unit - Experience the new functional unit for the 4D-NucleofectorTM System which expands our proven system to larger-scale transfection. The LV Unit allows for closed, scalable transfection of larger cell numbers in the range of 1x107 to 1x109 cells. Transfection protocols can be established in smaller scale using the X Unit and subsequently transferred to the LV Unit without the need for re-optimization. Transferability has been tested for various cell types, including human T cells, CHO-S, HEK293-S, or K-562.

There are cell type-specific Optimized Protocols or recommendations available in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.


Technical Specifications

Dimensions: (w x h x d): 24.5×20.7×28 cm / 9.7×4.1×11.0
Weight: 5.7 kg (12.5 lb)
Power: 100 - 240 VAC
Frequency: 50 - 60 Hz
Consumption: 0 VA (power off) / 20 VA (idle) / 140 VA (busy - during transfection)
Fuse: T 2.5A, L250V
Operating temperature: +15°C up to +40°C (59°F - 104°F)
Environnement non condensing Voltage limitation: 1500 V
Current limitation: 100 A


Quality statement: We are applying ISO9001:2015 standards for the design, manufacturing and testing of NucleofectorTM Devices, Solutions and Consumables since 2004.


  • Closed system: Sterile Nucleofection of up to 109 cells
  • Real scalability: Optimization in small scale 
  • Established protocols: Benefit from 700+ optimized cell types
  • Simple handling: Minimal training needs
  • 4D-NucleofectorTM LogWare: Optional operation via 21CFR part11 compliant software


  • Ex-vivo modification of human primary cells for cell therapy applications (e.g. genome editing, generation of CAR-T cells)
  • Transient production of potential therapeutic proteins or antibodies for construct screening
  • Generation of large numbers of transiently modified primary cells for cell-based assays

Content & Storage


1 Unit


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Transferability of NucleofectionTM Conditions from Small to Large Scale

Expression of maxGFPTM Protein in cells transfected in small volume (100 μL NucleocuvetteTM Vessels) or larger volumes (1 mL or LV NucleocuvetteTM Cartridge). Data represent the mean of various independent experiments.

Lonza GS XceedTM Cells transfected in small or larger scale show comparable IgG4 titers

For a human IgG4 antibody, similar titers of about 100 µg/mL were achieved in small scale (100 µl) and large scale transfections (1 ml fixed cartridge or 10 mL in flow through LV cartridge) in a 6-day batch culture without any feeds. IgG titers were increased by factor 2.5 when using higher seeding densities (not shown here). Switching to a 14-day fed-batch culture led to titers > 1 mg/mL (not shown here).

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Demo Request

4D-NucleofectorTM LV Unit

Are you interested in seeing our closed system for large-volume Nucleofection in your lab and how it performs for your cells? One of our transfection specialists can come to your lab and perform a transfection experiment with your cell type of interest.

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