AD1 4D-Nucleofector™ Y Kit
What You May Also Need
Product Overview
Electroporation-based methods have so far required cells to be in suspension for transfection. However, the 4D-NucleofectorTM Y Unit allows direct Nucleofection of cells in adherence. Cells which typically grow in adherence in cell culture, can be kept and transfected by Nucleofection in their physiological state.
The AD1 Primary Cell 4D-NucleofectorTM Y Kit is one of our two kits that are suited for electroporation of primary cells in adherence when working with the 4D-NucleofectorTM Y Unit. The cells are cultured in the 24-well culture plates provided with the kit* and for the transfection step a 24-well Dipping Electrode Array is inserted after replacing culture medium by the specific NucleofectorTM Solution mixed with the DNA or RNA substrate.
This kit has been determined to be optimal for transfecting following cell types:
- AoSMC (rat)
- Dorsal root ganglia DRG (rat)
- HUVEC (endothelial cells, umbilical vein, human)
- Neurons (mouse), cortex
- Neurons (rat), cortex
- Neurons (rat), hippocampal
- Osteoblast (rat)
- PAEC (endothelial cells, pulmonary artery, human)
- Retinal cells (rat)
For neurons or endothelial cells, please follow our respective Basic Protocols available under "Instructions" or in our knowledge database. For other cell types please refer to the Optimization Protocol for Primary Cells or Cell Lines.
* Other 24-well culture plates may not be compatible with the 24-well Dipping Electrode Array. Please contact our Scientific Support if you want to use alternative plates.
Benefits
- Nucleofection of neuronal cells at any time point during their culture period, i.e. at a later developmental stages
- Nucleofection of cells in adherence without the need for transfer in suspension
- Transfection efficiencies up to 70% combined with high viabilities
Applications
- Electroporation of cells in adherence
- For transfection of 2.5 x 104 - 1 x 105 cells
Content & Storage
Content
2 x 4.5 mL AD1 4D-NucleofectorTM Y Solution
2 x 1 mL Supplement 1
3 x 150 μg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 24-well Dipping Electrode Array
1 x NunclonTM Δ Surface 24-well plate (Nunc)