SG Cell Line 96-well Nucleofector™ Kit
What You May Also Need
Product Overview
The SG Cell Line 96-well ShuttleTM Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 96-well ShuttleTM Add-On.
This kit has been determined to be optimal for transfecting following cell types:
- Ba/F3
- Colo-205
- H4
- NIH/3T3
- Raji, Ramos
- THP-1
- WEHI
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.
Benefits
- High transfection efficiencies in difficult-to-transfect cell lines
- Compatible with liquid handling systems for automated high-throughput electroporation
- Conditions are transferable between 4D-NucleofectorTM System, 96-well ShuttleTM System and 384-well NucleofectorTM System
Applications
- Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
- High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries
Content & Storage
Content
2.25 mL SG Cell Line NucleofectorTM Solution
0.5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
1 x 96-well NucleocuvetteTM Plate (20 μl)
