Human Stem Cell NucleofectorTM Kit 1
The NucleofectorTM 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. The Human Stem Cell NucleofectorTM Kit 1 is one of our two kits suited for efficient transfection of primary human stem cells. Due to significant diversity between different human stem cell clones, we have developed two NucleofectorTM Kits (1 and 2) and an easy-to-use NucleofectorTM Starter Kit to determine which of the 2 kits is optimal for a specific clone.
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
- Low-throughput transfection in single cuvette format
Content & Storage
2 x 0.45 mL NucleofectorTM Solution for Human Stem Cells
2 x 0.1 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
10 Aluminum cuvette (100 µL)
10 Single use pipettes