Basic NucleofectorTM Kit for Primary Mammalian Neurons (SCN)
For transfection of mammalian neurons in small cell numbers (SCN) when using the NucleofectorTM IIS Device
Product Overview
This is a neural cell specific Basic NucleofectorTM Kit which allows to determine the optimal transfection conditions for any mammalian neuron type. In contrast to our standard Basic Kit (VPI-1003) this kit is suited for transfection of small cell numbers.
IMPORTANT NOTE: It only works in combination with an older NucleofectorTM Device model, the NucleofectorTM IIS Device
We offer an optimization protocol as well as specific pre-optimized protocols for
- Mouse DRGs
- Rat neurons
- Mouse neurons
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Benefits
- Efficient transfection of neural or glial cells in small scale (20 µL)
- Optimization can be performed within 1 experiment
- Detailed protocols guiding through the optimization procedure
- Optional result fine tuning with help of our Scientific Support Team
Applications
- Transfection of low cell numbers in the range of 2 - 5 x104 cells
- Kit suited for various neuron types from different mammalian species
Content & Storage
Content
0.45 mL Basic Neuron SCN NucleofectorTM Solution
0.1 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum SCN cuvettes (20 µL)
25 Single use pipettes
