Basic Nucleofector® Kit for Primary Mammalian Epithelial Cells
Basic Nucleofector® Kit for Primary Mammalian Epithelial Cells
For transfection of mammalian epithelial cells lacking a cell type specific Optimized Protocol for the Nucleofector® I/II/2b Device
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Product Overview
In addition to various cell type-specific kits for primary epithelial cells, we also provide a cell group-specific Basic Nucleofector® Kit. This kit is suited to determine the optimal transfection conditions for any mammalian epithelial cell type that lacks a cell type specific Optimized Protocol for the Nucleofector® 2b Device (or older generations I or II). In a first experiment a selection of different Nucleofector® Programs is tested in combination with the Basic Nucleofector® Solution for epithelial cells.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Request More InfoBenefits
- Optimization can be performed within 1 experiment
- Detailed protocol guiding through the optimization procedure
- Optional result fine tuning with help of our Scientific Support Team
- Transfection efficiencies up to 83%
- Viabilities up to 98%
Applications
- Applications - Kit suited for epithelial cells from different mammalian species and various organs
- Already tested for renal proximal tubular epithelial cells (RPTEC) human prostate epithelial cells (hPrEc) and human small airway epithelial cells (SAEC)
Storage and Content
2.25 mL Nucleofector® Solution for Primary Mammalian Epithelial Cells
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
