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Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 100 million, with cell population frequency

Name: Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 100 million, with cell population frequency
Brand: Lonza

Human Peripheral Blood Mononuclear Cells (hPBMC), Cryopreserved, 100 million, with cell population frequency

Catalog #: CC-2703P

Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 100 million cells with cell population frequency information

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1’054.20 CHF

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Product Overview

Lonza's Peripheral Blood Mononuclear Cells (PBMCs) are isolated from peripheral blood by apheresis and density gradient separation. Cells are collected from healthy donors following IRB protocols. They are guaranteed to contain ≥50 million viable cells per ampoule. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Mononuclear cell (MNC) rich cells from leukapheresis are depleted of RBCs and platelets. MNC cell profiles differ by donors and can contain on average around 50% T cells, 20% monocytes, 10% B cells, 10% NK cells and may contain up to 5% granulocytes. PBMCs of this part code have added characterization information available with population frequency values for T cells (CD3, CD4, CD8), B cells (CD19), Monocytes (CD14), NK cells (CD56), Dendritic cells (CD11c). With this part code you can now choose the donor that has the right percentage of the cell type of interest for your application.

Ability to Screen Different Donors

We maintain an inventory of different donors in an effort to satisfy your sample representation requirements. You can choose a lot based on donor demographic characteristics, and screen multiple lots to find the lot(s) that work best for you. In addition, we can request repeat donors if you need additional cells from a particular donor.

Rich Source of Immune Cells

Peripheral blood mononuclear cells (PBMC) are a mixed population of single nucleus cells. They can be further purified to isolate individual cell types such as natural killer cells, T cells, and B cells. In addition, PMBCs are a rich source of monocytes, which can be directed to differentiate into either macrophages or dendritic cells through culture with various cytokines. Researchers can choose X-Vivo 15 Hematopoeitic media that supports maintenance of lymphocytes and is optimized for serum-free culturing (additional cytokines may be necessary depending on application).

Please contact Scientific Support for serum containing media recommendations. Lonza guarantees the performance of Clonetics^TM/Poietics^TM cells only if appropriate Clonetics^TM/Poietics^TM media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

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Benefits

Test negative for mycoplasma, bacteria, yeast, and fungi
HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
Certificate of Analysis (CoA) is provided for each cell lot purchased

Applications

T -cell Proliferation assays
Cytokine release assays
ADCC assay
Immunogenicity studies
Cell therapy process development
Humanized mice
Biomarker discovery
Inflammatory studies
Immuno-oncology
CAR-T research

Storage and Content

1 x Cryopreserved ampule of Human Peripheral Blood Mononuclear Cells (hPBMC) containing ≥ 100 million cells

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SDS, CoA, and Instructions

Safety Data Sheets (SDS)

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Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Procedure for thawing Poietics™ cells

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Instructions for Use - Dendritic Cell (DC) and T Cell Assay from Matched PBMCs
Protocol for DC and T Cell Assay from Matched PBMCs

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Tips For Optimizing Your CD8+ T Cell and Dendritic Cell Co-cultures
A co-culture between matured dendritic cells (DCs) and CD8+ T cells isolated from Lonza cryopreserved human peripheral blood mononuclear cells (PBMCs; part nos. CC-2702 – CC-2705) and we present three major takeaways from this process that are critical for successful co-culture and end-point analysis for these two cell types

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Educational Material

Brochures, White Papers etc.

Videos

Procedure for thawing Poietics™ cells

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An Innovative, Highly Efficient Method for Transfecting Large Quantities of Primary Human T-cells
A strong need exists for improved cancer treatment options. Lonza's approach relies on genetically modified, patient-derived cells like T-cells or natural killer cells which express chimeric antigen receptors (CAR) targeting cancer cells.

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Discover the Freshness of Frozen PBMCs
An Assessment of Cryopreservation Impact on T Cell Activation

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Cryopreservation of Peripheral Blood Mononuclear Cells
Does Not Alter Immune Cell Phenotype

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Evaluating Performance of Cryopreserved PBMCs
Quality of Lonza PBMCS in comparison to competitors

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PBMCs in ADCC assay
Video showing functionality of PBMCs in ADCC assay.

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