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Pharma & Biotech

Human Mesenchymal Stem Cell (hMSC) Adipogenic Differentiation Medium BulletKitTM

Catalog #: PT-3004

Culture system containing basal medium and supplements required for maintenance and induction of hMSC adipogenic differentiation

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Product Overview

Stem cells possess the unique ability to differentiate into multiple lineages of cells including adipocytes. Lonza’s hMSC Adipogenic Differentiation BulletKitTM Medium is a serum containing media system designed to induce adipogenic differentiation of bone marrow derived hMSC into mature, functionally active adipocytes in as few as 19 days. Differentiation can then be validated by a variety of methods including the use of Lonza’s AdipoRedTM Adipogenic Assay Reagent which provides a quantitative value related to the amount of adipogenic differentiation.

Partial List of Cells Differentiated with hMSC Adipogenic Differentiation BulletKitTM Medium*:

  • Human bone marrow derived stem cells
  • C57BL/6 mice bone marrow derived stem cells
  • human adipose derived stem cells
  • Human periodontal ligament derived stem cells
  • Human hamstring tendon derived stem cells

The hMSC Adipogenic Differentiation BulletKitTM Medium provides both an induction medium and a maintenance medium guaranteed to induce adipogenic differentiation of bone marrow derived hMSC into mature, functionally active adipocytes; Each bottle of the hMSC Adipogenic Differentiation BulletKitTM Medium contains enough induction medium and maintenance medium to fully differentiate approximately 3 million bone marrow derived hMSC [approximately 15 wells in a 6-well plate format] into mature, functionally active adipocytes.

The hMSC Adipogenic Differentiation Medium is offered as a BulletKitTM Medium (catalog no. PT-3004) which includes both the basal media for induction and maintenance and the necessary supplements for both adipogenic induction and maintenance of bone marrow derived hMSCs.

Lonza guarantees the performance of cells only if appropriate media and reagents are used exclusively and the recommended storage and use protocols are followed. Any modifications made to the recommended cell systems including the use of alternative media, reagents or protocols, will void cell and media performance guarantees. If you need assistance in selecting the appropriate media, reagents, or protocol, please contact Lonza Scientific Support.

*List of cells generated from customer data. Lonza only routinely uses this medium with Lonza’s human bone marrow derived mesenchymal stem cells and can therefore only guarantee the successful use of this media with these cells.

Content & Storage

Content

1 x hMSC Adipogenic Maintenance Medium (PT-3102A)

1 x hMSC Adipogenic Induction Medium (PT-3102B)

1 x hMSC Adipogenic Maintenance SingelQuot Kit (PT-4122)

1 x hMSC Adipogenic Induction SingleQuot Kit (PT-4135)

 

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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