Basic Nucleofector™ Kit for Primary Mammalian Smooth Muscle Cells
Basic Nucleofector™ Kit for Primary Mammalian Smooth Muscle Cells
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Product Overview
In addition to various cell type-specific kits for primary smooth muscle cells, we also provide a cell group-specific Basic NucleofectorTM Kit. This kit is suited to determine the optimal transfection conditions for any mammalian smooth muscle cell type that lacks a cell type specific Optimized Protocol for the NucleofectorTM 2b Device (or older generations I or II). In a first experiment a selection of different NucleofectorTM Programs is tested in combination with the Basic NucleofectorTM Solution for smooth muscle cells.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Request More InfoBenefits
- Optimization can be performed within 1 experiment
- Detailed protocols guiding through the optimization procedure
- Optional result fine tuning with help of our Scientific Support Team
- Transfection efficiencies up to 95%
- Viabilities up to 96%
Applications
- Kit suited for smooth muscle cells from different mammalian species and various organs
- Already tested for porcine vascular smooth muscle cells and coronary artery smmoth muscle cells (CASMC)
Storage and Content
2.25 mL NucleofectorTM Solution for
Primary Mammaliean Smooth Muscle Cells
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
