Kinetic turbidimetric LAL assays take advantage of the exponential relationship between the appearance of the turbidity in the LAL test and the endotoxin concentration in the sample. This means that if a clot/gel forms (due to the endotoxin-specific enzymatic reactions of the LAL reagent), the time required for the turbidity change to occur is inversely proportional to the amount of endotoxin present. As such, by measuring the turbidity of the solution after certain periods of time using spectrophotometric methods, it is possible to reveal not just the presence of endotoxin but also the amount that is present.
These principles form the basis of our PYROGENTTM–5000 Kinetic Turbidimetric Assay, which offers a quantitative BET method with a sensitivity range from 0.01 to 100 EU/ml. The test sample is mixed with a LAL reagent that is specially reconstituted for this assay and placed in an incubating absorbance plate reader. It is then monitored over time for the appearance of turbidity (measured at the 340 nm wavelength). This assay is performed in a 96-well plate, which allows many samples to be processed at one time. As such, it is most effectively applied in laboratories needing to process large numbers of samples. It is also ideal for water samples, large volume parenterals, and water rinse from medical devices.
When using our kinetic turbidimetric LAL assay for bacterial endotoxin testing, our specially designed, fully integrated WinKQCLTM 5 Software can ensure the reliability and efficiency of your experiments, including automating data collection, management and reporting processes.