text.skipToContent text.skipToNavigation
Need help? Please contact us

P4 Primary Cell 96-well Nucleofector® Kit (960 RCT)

P4 Primary Cell 96-well Nucleofector® Kit (960 RCT)

Catalog #: V4SP-4960
For medium-throughput transfection of primary cells, e.g. mouse B-cells or immature DCs, when working with the 4D-Nucleofector® 96-well Unit.
Compare

5,825.00 EUR
SA

Product Overview

The P4 Primary Cell 96-well Nucleofector® Kit is one of our five kits suited for medium-throughput transfection of primary cells when working with the 4D-Nucleofector® 96-well Unit.

This kit has been determined to be optimal for transfecting following cell types:

  • B cell (mouse)
  • DC (mouse), immat.
  • MEF (mouse)

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

The kit is available in different variants for either 1 x 96 or 10 x 96 reactions.

Request More Info

Benefits

  • High transfection efficiencies for primary cells
  • Compatible with liquid handling systems for automated high-throughput electroporation
  • Conditions are transferable between 4D-Nucleofector® System, 4D-Nucleofector® 96-well Unit and 384-well Nucleofector® System

Applications

  • Transfection of low cell numbers in the range of 5 x 104 to 1 x 106 cells
  • High-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR libraries

Storage and Content

22.5 mL P4 Primary Cell Nucleofector® Solution
5 mL Supplement 1
50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
10 x 96-well Nucleocuvette® Plate (20 μl)

Return to Top

SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Return to Top

Educational Material

Brochures, White Papers etc.

  • The Nucleofector® 96-well Shuttle System - Nucleofection® of Cell Lines and Primary Cells in an Automated Framework

    Lonza WhitePaper
Return to Top

Subscribe to our eNewsletter

Keep up to speed on the latest scientific developments, events, tips and tools from Lonza.

Interested in our webinars?

Need a break from your daily routine? Grab a coffee and check out our free webinars. 

Knowledge Center

Need technical data?

Browse our Cell and Transfection, Citation, FAQ and Culture Media Technical Databases.