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Cell Line NucleofectorTM Kit R
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Catalog #: VVCA-1001
For efficient transfection of cell lines, e.g. HeLa, IMR-90, NIH/3T3, NK-92, or Sf9, in the NucleofectorTM I/II/2b Device
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Product Overview
The Cell Line NucleofectorTM Kit R is one of our five NucleofectorTM Kits suited for transfecting cell lines in the NucleofectorTM 2b Device. This kit has been determined to be optimal for transfecting following cell types:
- B16-F0
- BJ
- COS-7
- HeLa
- HT-29
- Hut-78
- IMR-90
- KG-1 L6
- LNCaP
- MDBK
- NIH/3T3
- NK-92
- PANC-1
- Sf9
- WI-38
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.
The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.Benefits
- High transfection efficiencies in difficult-to-transfect cell lines
Applications
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
- Low-throughput transfection in single cuvette format
Content & Storage
Content
4 x 2.25 mL NucleofectorTM Solution R
4 x 0.5 mL Supplement 1
4 x 30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
4 x 25 Aluminum cuvette (100 µL)
4 x 25 Single use pipettes
Instructions
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Safety Data Sheets (SDS)
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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