Rat Neuron Nucleofector™ Kit

Name: Rat Neuron Nucleofector™ Kit
Brand: Lonza

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Catalog #: VPG-1003

For efficient transfection of primary rat neurons in the Nucleofector^TM I/II/2b Device

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Overview

Product Overview

The Nucleofector^TM 2b Device (or older generations I or II) works with cell type specific kits, each of them dedicated to an individual primary cell. This Nucleofector^TM Kit is the optimal kit for efficient transfectiion of primary rat neurons.

The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.

Benefits

High transfection efficiencies for primary cells
Cells maintain functionality post transfection

Applications

Applications - Transfection of cell numbers in the range of 2 × 10⁵ to 2 × 10⁷ cells
Low-throughput transfection in single cuvette format

Content & Storage

Content

2.25 mL Nucleofector^TM Solution for Rat Neurons
0.5 mL Supplement 1
30 µg pmaxGFP^TM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes

Instructions, SDS, CoA