Human CD19+ B Cells (10 Million Cells, Positive Selection)
Human CD19+ B cells isolated from peripheral blood using positive immunomagnetic selection, cryopreserved, ≥10 million cells
Do you need a modified version of this cell product, such as different vialing sizes, matched immune cell isolations or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research
What You May Also Need
Product Overview
Leukapheresed CD19+ B Cells are positively selected from leukapheresed peripheral blood MNCs using immunomagnetic CD19 microbeads. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols.
Applications
- Generating antibody libraries
- Co-culture with T helper cells or cytotoxic lymphocytes (CTL)
- B cell activation, clonal expansion, Ig rearrangement, and Ig secretion
- Antigen presenting cell (APC) signal transduction and antigen presentation
Content & Storage
Content
1 vial containing ≥ 10 million cryopreserved cells