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Pharma & Biotech

Human CD19+ B Cells (10 Million Cells, Negative Selection)

Catalog #: 4W-601
Human CD19+ B cells isolated from peripheral blood using negative immunomagnetic selection, cryopreserved, ≥10 million cells
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Product Overview

Leukapheresed CD19+ B cells are negatively selected from leukapheresed peripheral blood MNCs using an immunomagnetic labeling system to remove unwanted cell types leaving the B cells untouched. Cells are cryopreserved in Lonza Cryo Media in a cryovial. Cell purity is determined post thaw using a FACS assay. Count and viability is determined using AO/PI. Cells are collected from healthy donors following IRB protocols.

Applications

  • Generating antibody libraries
  • Co-culture with T helper cells or cytotoxic lymphocytes (CTL)
  • B cell activation, clonal expansion, Ig rearrangement, and Ig secretion
  • Antigen presenting cell (APC) signal transduction and antigen presentation

Content & Storage

Content

1 vial containing ≥ 10 million cryopreserved cells

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

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