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Pharma & Biotech

X-VIVOTM 10 Serum-free Hematopoietic Cell Medium

Catalog #: BE04-380Q

X-VIVOTM 10, Serum free hematopoietic cell medium, with L-Glutamine, gentamicin and phenol red, xenofree, 1 L

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Product Overview

The X-VIVOTM 10 Media formulations are designed to support the generation of LAK cells in a serum-free environment. The original protocols involved the incubation of patient or normal donor peripheral blood lymphocytes (PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK cell generation is achieved when peri pheral blood lymphocytes are incubated for 3 – 10 days at a density of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 Cetus units of rIL-2.

X-VIVOTM 10 Media is available as a 1X liquid in two convenient formulations. X-VIVOTM 10, catalog number BE04-380Q contains L-glutamine, gentamicin, and phenol red. Catalog number BE04-743Q contains L-glutamine but does not contain gentamicin or phenol red. Catalog number BE02-055Q contains L-glutamine and recombinant transferrin but does not contain gentamicin or phenol red. The recombinant transferrin replaces native transferrin. 

  L-Glutamine Gentamycin Phenol Red Transferrin
BE04-380Q  +  +  +   +
BE04-743Q  +       +
BE02-055Q  +       +(recombinant)

 

All BioWhittakerTM Cell Culture Media products are for Research Use Only (RUO) and are not approved for human or veterinary use or for use in clinical or in vitro diagnostic procedures. If you require GMP grade media, contact Lonza for more details.

Benefits

  • Superior media for most hematopoietic cells types including dendritic cells, lymphocytes, monocytes, macrophages and granulocytes.
  • Formulations only contain human derived components
  • More regulatory friendly as human component is replaced by a recombinant one
  • Highly cited in scientific literature
  • Clinical proven formulations providing process safety and constancy
  • High productivity resulting in a high viable cell yield
  • Serum Free media result in a more defined process and result in limited immune response
  • Efficient transgene expression Desirable T-cell phenotype

Applications

  • Proliferation of peripheral blood lymphocytes
  • Proliferation of tumor infiltrating lymphocytes
  • Cryopreservation of human tissue
  • Cultivation of human monocytes and macrophages
  • Cultivation of stem cells
  • Cultivation of dendritic cells

Content & Storage

Content

1L bottle

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

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