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Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons

Rat Embryonic Dorsal Root Ganglion (eDRG) Neurons

Catalog #: R-EDRG-515
Cryopreserved Rat Embryonic Dorsal Root Ganglion ampule containing ≥ 1,000,000 cells
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 1’664.00 CHF
AMP

Product Overview

Ready to use Rat Embryonic Dorsal Root Ganglion (DRG) Neurons are suspensions of high quality sensory neurons prepared by standardized methods, and are ready for immediate culture.

Each vial of Embryonic Dorsal Root Ganglion Neurons contains approximately 1 million cells in 0.25 ml suspension. Some cell death will occur during the first few days after plating and debris will be observed. This is normal. After approximately 4 days in culture, the cells will form a neurite network and by the 7th day, debris will be minimal.In the absence of mitotic inhibitors, the neurons tend to cluster (ganglionate) and detach from the substrate.

Rat Embryonic Dorsal Root Ganglion Neurons stain positive for Tuj. Primary neuronal cells need an appropriate substrate to adhere and survive. The preferred substrate is poly-D-lysine with laminin. Poly-D-lysine can also be used alone to coat the cell culture plasticware or cover slips. Coated cell culture plates, dishes, or cover slips can either be purchased from a supplier or prepared immediately prior to use.

Recommended medium: PNGMTM BulletKitTM with addtion of 100 ng/ml of Nerve Growth Factor-β from rat (Sigma No. N2513)*  

*IMPORTANT: For the survival and growth of embryonic DRG neurons, the addition of 100 ng/ml of Nerve Growth Factor to the media is required at all times (Sigma No. N2513). For inhibition of Schwann cell proliferation, mitotic inhibitors will also need to be added.

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Benefits

  • Ready-To-Use 
  • No tissue dissection or animal handling required 
  • Increased data reproducibility
  • Characteristic morphology and staining tests confirm tissue origin and cell type
  • Negative for mycoplasma, bacteria, yeast and fungi
  • Certificate of Analysis (CoA) is available for every batch 

Applications

  • Neurogenesis
  • Neurotransmitter Function
  • Gene Expression
  • Signaling Pathways
  • Electrophysiology
  • Neurotoxicity
  • Drug/Compound Screening
  • Advanced Cell Culture Models

Storage and Content

  • ≥ 1,000,000 cryopreserved cells per ampule
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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Supplemental Inst. - Neuronal Plate Coating Instructions

    Instructions of cell culture plate coating procedure required for successful neuronal culture
  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
  • Instructions - Rat Embryonic DRG Neuronal Cell System

    Instructions for proper use and culture of Rat Embryonic Dorsal Root Ganglion Neurons
  • TechSheet - Rat Embryonic DRG Neuronal Cell System

    Technical tips for culturing Rat Embryonic Dorsal Root Ganglion Neurons
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Educational Material

Brochures, White Papers etc.

  • Cryopreserved Neural Cells and Media

    Lonza's Cryopreserved Neurons Offer Comparable Results to Fresh Cells
  • Quality and Time: The Real Costs of Your Animal Neural Cell Isolation

    Lonza infographic illustrating the benefits of using Lonza's Ready-to-Use Cells
  • Primary Cells and Media Reference Guide

    A comprehensive collection of Lonza primary cell and media products supporting many research areas.
  • Cell List – Integrated Solutions for Primary Cells and Transfection

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