ViaLight® Plus Cell Proliferation and Cytotoxicity BioAssay Kit, 500 Test
ViaLight® Plus Cell Proliferation and Cytotoxicity BioAssay Kit, 500 Test
ViaLight® Plus, 500 Tests
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Product Overview
ViaLight® Plus BioAssay Kits are designed to provide unprecedented speed and sensitivity for cytotoxicity and cell proliferation studies. The ViaLight® Assay incorporates bioluminescent detection of cellular ATP as a measure of viability. Measurement of ATP is the most accurate, effective, and direct way of determining the number of living cells in culture.
The ViaLight® Plus BioAssay is designed to deliver a high, stable luminescent signals for an extended period of time for greater experimental design flexibility. The easy two-step, non-radioactive assay is scalable for high-throughput applications in both 96-well and 384-well formats. The assay can be run on a variety of luminometers.
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Benefits
- Fast - Results from a 96 well plate can be processed and analyzed in as little as 10 minutes.
- Sensitive - Detect as few as 10 cells allowing for lower seeding densities and more assays
- Convenient - Simply add two reagents directly to your culture well and read. No cell washing or medium removal required.
- Flexible - Dynamic range of 5 decades and excellent performance with both adherent or suspension cultures allows you to design the experiment for your needs.
- Robust - Stable bioluminescent signal (half-life >5 hours) facilitates manual or batch processing. And, unlike MTT or Alamar Blue assays, ViaLight® Bioassays are not prone to interference due to culture condition changes.
Storage and Content
500 test kit (Sufficient for 5 plates)
- 1 x LT27-212 ATP monitoring reagent plus, Lyophilized
- 1 x LT27-079 Assay buffer, 50 mL bottle,
- 1 x LT27-076 Cell lysis reagent, 50 mL bottle
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Instructions - ViaLight® Plus Kit
Instructions for proper use of the ViaLight® Plus Kit -
Luminescent Cell-Based Assays
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Long-Term Culture of Primary Human Hepatocyte 3D Spheroids and Cytotoxicity Assay
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Determination of Spheroid Viability and Proliferation Using the ViaLight® Plus Cell Proliferation and Cytotoxicity BioAssay
Lonza's ViaLight® Plus Luminescent Cell Viability BioAssay can be used with spheroid 3D cell cultures with only slight modifications of the protocol. -
Stem Cell Research Tools for Biologically Relevant Results
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Measuring Hepatotoxicity in Primary Human Hepatocyte 3D Spheroid Using ViaLight Plus Cytotoxicity BioAssay
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Educational Material
Brochures, White Papers etc.
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Assessment of the Anti-angiogenic Effect of VEGFR2 siRNA in HUVEC
Scientific poster for AACR 2015 -
ViaLight® Plus Cell Proliferation Assay vs. MTT
Informational product application note -
Luminescent Cell-Based Assays
Learn more about Lonza's luminecent cell-based assay offerings -
Cell-based Assays Using Hard-to-transfect Cell Lines and Transiently Transfected Primary Cells
Co-sponsered poster with Promega -
Development of a Long-term Primary Hepatocyte 3D Spheroid Model
For Use in DILI Applications using ViaLight Plus Cytotoxicity BioAssay Kit -
Analyzing Cell Viability in 3D Tissue Models with the ViaLight™ Plus BioAssay
Learn how Lonza's ViaLight™ Plus BioAssay can be used to assess cell health in 3D culture -
Advanced hepatic cell culture techniques
Drug-Induced Liver Injury (DILI) toxicology modeling throughout the discovery pipeline
ViaLight® Plus signal stability competitor comparison
A study of ViaLight® Plus signal stability compared to a leading competitor
ViaLight® Plus signal stability data
ViaLight® Plus BioAssay Kit signal stability data
