Basic Parasite Nucleofector™ Kit 1
For efficient transfection of parasites, e.g. Plasmodium berghei or Trypanosoma brucei, in NucleofectorTM 2b Device
This Nucleofector® Kit size is no longer available. Please choose a different size from the menu below.
What You May Also Need
Product Overview
The Basic Parasite NucleofectorTM Kit 1 is one of our two NucleofectorTM Kits suited for transfecting parasites, e.g. Plasmodium berghei or Trypanosoma brucei, in NucleofectorTM 2b Device. Parasitic protozoa infect vertebrates and invertebrates and some are even parasitic in plants. In humans, they can cause severe diseases, such as Malaria (Plasmodium), Sleeping Sickness (Trypanosoma) or Leishmaniasis (Leishmania). Nucleofection has proven to provide considerably higher transfection efficiencies (e.g., in Plasmodium berghei and Trypanosoma brucei) compared to standard methods, such as electroporation or particle bombardment. Due to significant genotypic and phenotypic diversity between species and life cycles, we have developed two Basic Parasite NucleofectorTM Kits (1 and 2) and an easy-to-use Basic Parasite NucleofectorTM Starter Kit to determine which of the 2 kits is optimal for a specific parasite type. The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
Benefits
- Increased transfection efficiencies compared to standard methods, such as electroporation or particle bombardment
Applications
- Proven results for e.g. Plasmodium berghei and Trypanosoma brucei
Content & Storage
Content
2 x 0.45 mL NucleofectorTM Basic Solution for Parasites
2 x 0.1 mL Supplement 1, 30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
10 Aluminum cuvette (100 µL)
10 Single use pipettes
