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Pharma & Biotech

HUVEC - Human Umbilical Vein Endothelial Cells without VEGF, Single Donor, in EGM™-Plus

Catalog #: CC-2935

Cryopreserved ampule of Human Umbilical Vein Endothelial Cells (HUVEC) without VEGF, from a single donor, cultured in EGMTM Plus and containing ≥ 500,000 cells 


Product Overview

HUVECs cultured in EGMTM-Plus Growth Media offer faster proliferation rates while maintaining the same high quality characterization as HUVECs in EGMTM Media 

Lonza's Cryopreserved HUVECs are shipped as frozen primaries. They are guaranteed to express CD31/105 and survive in culture through 15* population doublings (*except HUVEC-XLTM guaranteed through 5 PD).

All cells test negative for mycoplasma, bacteria, yeast, and fungi. HIV-1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots. A Certificate of Analysis is provided for each cell lot purchased.

Our pooled vs. single donor options allow researchers to study or exclude donor variability from their experiments. Depending upon the study, pooled version is typically utilized to reduce donor variability while single donor option allows researchers to assess donor-specific differences.

HUVEC-XL size consists of 10 million cells/amp achieved by pooling donors. This option is designed to support studies that require large volume of cells while still offering cost-benefits to researchers.

Not All HUVECs Are Created Equal – Which Type Is Suitable for Your Research?

  • CC-2935 (single donor) - Isolated inEGMTM - Plus Media: Improved version of part number CC-2517. Offers faster proliferation rates than HUVEC isolated in EGMTM Media. No exogenous VEGF.
  • CC-2517 (single donor), CC-2519 (pooled donor) - Both isolated in EGMTM Media, in absence of defined growth factors. Contains BBE. No exogenous VEGF.
  • C2517A (single donor), C2519A (pooled donor), 00191027 (XL size) - Isolated in EGMTM-2 Media, in presence of growth factors such as VEGF, rhFGF, rhEGF, and others. Does not contain BBE.
  • C2517AS (single donor), C2519AS (pooled donor) - Pre-screened HUVECs in EGMTM-2 Media. Screened for angiogenesis markers and test positive for expression of eNOS, Axl, Tie-2 and VEGFr2.



  • Guaranteed through 15 population doublings 
  • Test negative for mycoplasma, bacteria, yeast, and fungi
  • HIV-1, Hepatitis B and Hepatitis C are not detected for all donors and/or cell lots
  • A Certificate of Analysis is provided for each cell lot purchased



  • Would healing
  • Angiogenesis
  • Tissue engineering
  • Inflammation
  • Oncology
  • Pharmacology
  • Vascular modeling
  • Transfection 


Content & Storage


1 x Cryopreserved ampule HUVEC containing ≥ 500,000 cells/vial


Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

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