KGM™ Gold Keratinocyte Growth Medium BulletKit™, Calcium Free
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Product Overview
A new robust and versatile serum-free medium formulation for high and low density NHEK-Normal Human Epidermal Keratinocyte cultures.
KGM-GoldTM Calcium Free Keratinocyte Growth Medium is designed to support both clonal growth and high density keratinocyte proliferation. The medium supports superior growth and morphology of keratinocytes.
Furthermore, quality control at Lonza requires that keratinocytes pass strict morphological criteria specifying the minimal presence of enlarged and vacuolated cells, enabling Lonza keratinocytes to be used by the researcher for ≥15 Population Doublings.
Content & Storage
Content
1 x Bottle Basal Medium 500 mL
1 x Natural Cap Vial with Hydrocortisone 0.50 mL
1 x Natural Cap Vial with Transferrin 0.50 mL
1 x Amber Vial with Epinephrine 0.25 mL
1 x Red Cap Vial with GA-1000 0.50 mL
1 x Orange Cap Vial with BPE 2.00 mL
1 x Green Cap Vial with hEGF 0.50 mL
1 x Lilac Cap Vial with Insulin 0.50 mL
Instructions
Safety Data Sheets (SDS)
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.Morphology of Lonza’s Adult Keratinocytes Grown in KGM™ Gold Growth Medium Compared to Competitor A’s Adult Keratinocytes in Their Medium.
The growth performance and longevity of Competitor A's adult keratinocytes grown in Competitor A's Medium were compared with Lonza’s adult keratinocytes grown in KGM-Gold™ Growth Medium. Although Competitor A claims ≥25 total population doublings for their adult keratinocytes, actual results show that this lot barely obtained 19 PD. Note the presence of several enlarged cells in Passage 2 in Competitor’s Medium. At the end of passage 4 (15 PD) Lonza’s adult keratinocytes consist of mostly small proliferative cells.