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Pharma & Biotech

MEGMTM Mammary Epithelial Cell Growth Medium BulletKitTM

Catalog #: CC-3150

Culture system containing MEBMTM Basal Medium (CC-3151) and MEGMTM SingleQuotsTM Supplements (CC-4136) required for growth of Mammary Epithelial Cells.

 

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Product Overview

MEGMTMBulletkitTM, is also sold as separate basal medium and supplements.

MEGMTMTM Mammary Epithelial Cell Growth Medium has been optimized for the growth of Mammary Epithelial cells in a serum-free environment. This media system has been referenced in over 130 unique publications primarily focusing on breast cancer research. 

Did you know our customers have used MEGMTMTM BulletKitTM not only for Lonza's primary HMECs but also to culture well-established normal breast cell lines such as MCF-10A and MCF-12A? 

MEGMTMTM BulletKitTM is optimized and readily available media kit giving researchers the confidence and the convenience of a system that works to support their cells. This is especially important for researchers who are utilizing normal breast cell lines as a stepping stone to primary HMEC cell culture. Working with the same media between cell lines and primary cells can reduce variability within the experiments and simplifies the shift for researchers. In addition, if primary HMECs are utilized as a side-by-side control with normal breast cell lines, the MEGMTMTM BulletKitTM media may also offer cost-benefits since customers can use the same media to support both cell types.

Purchase our Mammary Epithelial Cell Culture Kit (catalog no. CC-2551B) and get a discounted price on cells and media. The kit consists of a cryopreserved ampule of mammary epithelial cells (catalog no. CC-2551) and MEGMTMTM BulletKitTM Growth Media (catalog no. CC-3150)

 

 

  

Content & Storage

Content

1 x MEBMTM Basal Medium (CC-3151), 500 mL

1 x MEGMTM SingleQuotsTM Supplement Pack (CC-4136) containing:

1 x Orange Cap Vial with BPE, 2.00 mL

1 x Green Cap Vial with hEGF, 0.50 mL

1 x Lilac Cap Vial with Insulin, 0.50 mL

1 x Natural Cap Vial with Hydrocortisone, 0.50 mL

1 x Red Cap Vial with GA-1000, 0.50 mL

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Lonza’s HMEC cells cultured in MEGMTM BulletKitTM Medium vs. other commercially available systems

Graph showing the propagation potential of Lonza’s HMEC cells cultured in MEGMTM BulletKitTM Medium vs. other commercially available systems

A Three-Dimensional RAFTTM Co-Culture as Advanced Model for Breast Cancer Drug Discovery

Presented at AACR 2017, we demonstrate here the feasibility of co-culturing human mammary fibroblasts and human mammary epithelial cells in RAFTTM 3D Culture System as a 3D breast cancer model.

Selected publications out of many where our customers have used MEGM™ Growth Media with various normal breast cells.

  • Savanur, et al. shows Lonza's HMECs were cultured in Lonza's MEGM™ Media alongside breast cancer cell lines in the respective media to understand the inhibitory effects of Lectin on proliferation of breast cancer cells
  • Park, et. al. PLoS ONE 10(6) demonstrates how MCF-10A and MCF-12A were cultured in MEGM™™ alongside breast cancer cell lines in their respective media to examine the effects of cold atmospheric plasma on epigenetic alterations
  • Kmiecik, et al. 2015. PLoS ONE 10(5) shows MEGM™ Medium's compatibility with immortalized hTERT-HME1 cells in a study of MT3-overexpression on proliferation, invasiveness, migration,and tumorigenesis of breast cancer cells

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