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PPiLight™ Inorganic Pyrophosphate Assay, 500 Test
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Catalog #: LT07-610
PPiLightTM Assay, 500 Tests
Product Overview
Inorganic pyrophosphate (also referred to as diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number of different enzymatic reactions. Enzymes that utilize PPi as a substrate may include phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymes that generate PPi are more numerous and may include cyclases (EC 4.6.1), hydrolyses (EC 3), ligases (EC 6) and DNA polymerases such as DNA Polymerase theta (θ) encoded by POLQ.
The PPiLightTM Assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate. In the presence of PPi the detection reagent catalyses the conversion of AMP to ATP. The assay uses luciferase, which produces light from the newly formed ATP and luciferin.
Benefits
- Fast - Measure enzyme activity via pyrophosphate consumption in 1 hour
- Simple - The 2 step, luminescent assay doesn’t require radioactive substrates, beads, detection antibodies or modified substrates
- Wide Detection Range - Sensitive to 0.02 µM with a linear range to 10 µM
- Versatile - Scalable to 96-, 384-, 1586-well formats
Applications
Measure activity of:
- Phosphotransferases
- Pyrophosphotases
- Cyclases
- Ligases
- Hydrolases
- DNA Polymerases
Content & Storage
Content
- 1 x LT27-263 PPiLightTM converting reagent, 10 mL
- 1 x LT27-260 PPiLightTM detection reagent, 10 mL
- 1 x LT27-266 PPiLightTM reconstitution buffer, 20 mL
Storage Conditions: 2°C - 8°C
Instructions
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