Stellate Cells, human, passage 1
Human Cryopreserved Stellate Cells, 1 million cells per vial, passage 1
What You May Also Need
Stellate cells are a resident cell type of the liver primarily functioning to store retinoids. In response to liver damage, stellate cells rapidly lose the stored retinoids and differentiate into a proliferating fibroblast-like cell that begins depositing collagen matrix. This activity causes buildup of collagen in the liver eventually leading to cirrhosis.
Stellate cells can be isolated from liver tissue, enriched, and placed into cell culture. When placed into culture, stellate cells begin to immediately differentiate into the activated state.
Human Cryopreserved Stellate Cells, 1 million cells/vial, are stellate cells enriched from human liver isolations and grown to >80% confluency and then cryopreserved.
Human Stellate Growth Medium (Catalog MCST250) is available to support optimal culture health and growth characteristics of stellate cells.
- Post-thaw plating to observe characteristic stellate morphology.
- Flow cytometry using antibodies for Vinculin, Vimentin, and α-SMA
- Low passage for greater activation potential
- Used in co-cultures with primary hepatocytes
Content & Storage
- >1,000,000 cells/vial