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PPiLight™ Inorganic Pyrophosphate Assay, 500 Test

PPiLight™ Inorganic Pyrophosphate Assay, 500 Test

Catalog #: LT07-610
PPiLightTM Assay, 500 Tests
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 381.00 CHF
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Product Overview

Inorganic pyrophosphate (also referred to as diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number of different enzymatic reactions. Enzymes that utilize PPi as a substrate may include phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymes that generate PPi are more numerous and may include cyclases (EC 4.6.1), hydrolyses (EC 3), ligases (EC 6) and DNA polymerases such as DNA Polymerase theta (θ) encoded by POLQ. 
 
The PPiLightTM Assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate. In the presence of PPi the detection reagent catalyses the conversion of AMP to ATP. The assay uses luciferase, which produces light from the newly formed ATP and luciferin.
 

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Benefits

  • Fast - Measure enzyme activity via pyrophosphate consumption in 1 hour
  • Simple - The 2 step, luminescent assay doesn’t require radioactive substrates, beads, detection antibodies or modified substrates
  • Wide Detection Range - Sensitive to 0.02 µM with a linear range to 10 µM
  • Versatile - Scalable to 96-, 384-, 1586-well formats

Applications

Measure activity of:

  • Phosphotransferases
  • Pyrophosphotases
  • Cyclases
  • Ligases
  • Hydrolases
  • DNA Polymerases

Storage and Content

  • 1 x LT27-263 PPiLightTM converting reagent, 10 mL
  • 1 x LT27-260 PPiLightTM detection reagent, 10 mL
  • 1 x LT27-266 PPiLightTM reconstitution buffer, 20 mL

Storage Conditions: 2°C - 8°C

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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Instructions – PPiLight™ Inorganic Pyrophosphate Assay Kit

    Instructions for proper use of PPiLight™ Inorganic Pyrophosphate Assay Kit
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