Product Overview
The SF Cell Line 4D-Nucleofector® LV Kit XL* is suited for larger scale transfection of cell lines in the LV Nucleocuvette® Cartridge format when working with the 4D-Nucleofector® LV Unit. With the LV Nucleocuvette® Cartridge a variable volume of up to 20 mL can be transfected. The cartridge is automatically filled and emptied with help of the supplied input and output reservoirs. Alternatively bags can be used. An optional second input allows for separate feeding of mRNA to avoid its degradation.
This kit has been determined to be optimal for transfecting following cell lines and many others that use Solution SF as optimal Nucleofector® Solution:
- HEK293 suspension clones
- CHO-S suspension clones
- K-562
- U-937
The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.
*Note: From the three cell line solutions (SE, SF, SG) that come with the 4D-Nucleofector® System only SF is available for the large-scale formats so far. Solution SF is suited for the vast majority of cell lines that might be used in larger scale applications (e.g. suspension CHO or 293 cells). For other solutions please contact our Scientific Support.
Request More InfoBenefits
- Closed Nucleofection of up to 109 cell lines
- Conditions transferable from existing smaller scale protocols
- Automatic processing via tubing system
- Transfection of up to 20 mL reaction volume in less than 10 minutes
Applications
- For large scale transfection of up to 1 x 109 cells
- Transient production of potential therapeutic proteins or antibodies for construct screening
- Generation of large numbers of transiently modified cell lines for cell-based assays
Storage and Content
5 x 22.5 mL SF Cell Line Nucleofector® Solution
5 x 5 mL Supplement 1
5 x LV Nucleocuvette® Cartridge
10 x 4D-Nucleofector® LV Reservoir
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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4D-Nucleofector® Protocol for TF-1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for TF-1 -
4D-Nucleofector® Protocol for A-375
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for A-375 -
4D-Nucleofector® Protocol for CCRF-CEM
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for CCRF-CEM -
4D-Nucleofector® Protocol for C6 Glioma
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for C6 Glioma -
4D-Nucleofector® Protocol for L428
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for L428 -
4D-Nucleofector® Protocol for FDC-P1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for FDC-P1 -
4D-Nucleofector® Protocol for CHO-K1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for CHO-K1 -
4D-Nucleofector® Protocol for EL4
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for EL4 -
4D-Nucleofector® Protocol for K562
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for K562 -
4D-Nucleofector® Protocol for HL-60
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HL-60 -
4D-Nucleofector® Protocol for RIN-m5F
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for RIN-m5F -
4D-Nucleofector® Protocol for PC-12
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for PC-12 -
4D-Nucleofector® Protocol for S2
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for S2 -
4D-Nucleofector® Protocol for Molt-4
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Molt-4 -
4D-Nucleofector® Protocol for SK-N-AS
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for SK-N-AS -
4D-Nucleofector® Protocol for J774A.1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for J774A.1 -
4D-Nucleofector® Protocol for H9c2(2-1)
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for H9c2(2-1) -
4D-Nucleofector® Protocol for HEK-293 sus
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HEK-293 sus -
4D-Nucleofector® Protocol for AGS
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for AGS -
4D-Nucleofector® Protocol for HMC-1-8
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HMC-1-8 -
4D-Nucleofector® Protocol for S49
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for S49 -
4D-Nucleofector® Protocol for HT-29
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HT-29 -
4D-Nucleofector® Protocol for Vero
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Vero -
4D-Nucleofector® Protocol for SH-SY5Y
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for SH-SY5Y -
4D-Nucleofector® Protocol for NCI-H1299
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for NCI-H1299 -
4D-Nucleofector® Protocol for P19
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for P19 -
4D-Nucleofector® Protocol for A20
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for A20 -
4D-Nucleofector® Protocol for Neuro-2a
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Neuro-2a -
4D-Nucleofector® Protocol for NS0
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for NS0 -
4D-Nucleofector® Protocol for KG-1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for KG-1 -
4D-Nucleofector® Protocol for A549
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for A549 -
4D-Nucleofector® Protocol for T2
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for T2 -
4D-Nucleofector® Protocol for HEK-293
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HEK-293 -
4D-Nucleofector® Protocol for A431
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for A431 -
4D-Nucleofector® Protocol for MDBK
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MDBK -
4D-Nucleofector® Protocol for SK-BR-3
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for SK-BR-3 -
4D-Nucleofector® Protocol for Sp20-Ag14
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Sp20-Ag14 -
4D-Nucleofector® Protocol for COS-1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for COS-1 -
4D-Nucleofector® Protocol for Sf9
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Sf9 -
4D-Nucleofector® Protocol for PC-3
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for PC-3 -
4D-Nucleofector® Protocol for T84
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for T84 -
4D-Nucleofector® Protocol for U266B1
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for U266B1 -
4D-Nucleofector® Protocol for SK-N-MC
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for SK-N-MC -
4D-Nucleofector® Protocol for Hut 78
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Hut 78 -
4D-Nucleofector® Protocol for RBL-2H3
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for RBL-2H3 -
4D-Nucleofector® Protocol for NALM-6
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for NALM-6 -
4D-Nucleofector® Protocol for CHOsus
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for CHOsus -
4D-Nucleofector® Protocol for MDA-MB-453
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for MDA-MB-453 -
4D-Nucleofector® Protocol for SK-OV-3
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for SK-OV-3 -
4D-Nucleofector® Protocol for LNCaP
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for LNCaP -
4D-Nucleofector® Protocol for HepG2
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HepG2 -
4D-Nucleofector® Protocol for HT-1080
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for HT-1080 -
4D-Nucleofector® Protocol for SK-N-SH
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for SK-N-SH -
4D-Nucleofector® Protocol for Saos-2
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for Saos-2 -
4D-Nucleofector® Protocol for IMR32
Optimized 384-well Nucleofector® and 4D-Nucleofector® Protocol for IMR32
Lonza GS Xceed™ Cells transfected in small or larger scale show comparable IgG4 titers
For a human IgG4 antibody, similar titers of about 100 µg/mL were achieved in small scale (100 µl) and large scale transfections (1 ml fixed cartridge or 10 mL in flow through LV cartridge) in a 6-day batch culture without any feeds. IgG titers were increased by factor 2.5 when using higher seeding densities (not shown here). Switching to a 14-day fed-batch culture led to titers > 1 mg/mL (not shown here).