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Pharma & Biotech

SF Cell Line 4D-NucleofectorTM LV Kit L

Catalog #: V4LC-2002
For larger scale transfection of cell lines, e.g. suspension CHO or HEK293, in the 1 mL NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
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Product Overview

The SF Cell Line 4D-NucleofectorTM LV Kit L* is suited for larger scale transfection of cell lines in the 1 mL NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit. With the 1 mL NucleocuvetteTM Cartridge a fixed volume of 1 mL can be transfected. The cartridge is manually filled via a sterile injection port.

This kit has been determined to be optimal for transfecting following cell lines and many others that use Solution SF as optimal NucleofectorTM Solution:

  • HEK293 suspension clones
  • CHO-S suspension clones
  • K-562
  • U-937

The respective cell type-specific Optimized Protocols or recommendations are available under "Instructions" or in our knowledge database. In these Optimized Protocols the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of individual cell types.

 

*Note: From the three cell line solutions (SE, SF, SG) that come with the 4D-NucleofectorTM System only SF is available for the large-scale formats so far. Solution SF is suited for the vast majority of cell lines that might be used in larger scale applications (e.g. suspension CHO or 293 cells). For other solutions please contact our Scientific Support.

Benefits

  • Closed Nucleofection of up to 108 cells
  • Conditions transferable from existing smaller scale protocols
  • Simple manual filling via sterile injection port
  • Transfection with seconds

 

Applications

  • For large scale transfection of up to 1 x 108 cells
  • Transient production of potential therapeutic proteins or antibodies for construct screening
  • Generation of large numbers of transiently modified cell lines for cell-based assays

 

Content & Storage

Content

2.25 mL SF Cell Line NucleofectorTM Solution

0.5 mL Supplement 1

50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)

2 x 1 mL NucleocuvetteTM Cartridge

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

Transferability of NucleofectionTM Conditions from Small to Large Scale

Expression of maxGFPTM Protein in cells transfected in small volume (100 μL NucleocuvetteTM Vessels) or larger volumes (1 mL or LV NucleocuvetteTM Cartridge). Data represent the mean of various independent experiments.

Lonza GS XceedTM Cells transfected in small or larger scale show comparable IgG4 titers

For a human IgG4 antibody, similar titers of about 100 µg/mL were achieved in small scale (100 µl) and large scale transfections (1 ml fixed cartridge or 10 mL in flow through LV cartridge) in a 6-day batch culture without any feeds. IgG titers were increased by factor 2.5 when using higher seeding densities (not shown here). Switching to a 14-day fed-batch culture led to titers > 1 mg/mL (not shown here).

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