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P2 Primary Cell 96-well Nucleofector® Kit
Catalog #: V4SP-2096
For medium-throughput transfection of primary cells, e.g. mouse macrophages or NHDF, when working with the 4D-Nucleofector® 96-well Unit.
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P5 Primary Cell 96-well Nucleofector® Kit (960 RCT)
Catalog #: V4SP-5960
For medium-throughput transfection of primary cells, e.g. HUVEC, when working with the 4D-Nucleofector® 96-well Unit.
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P5 Primary Cell 96-well Nucleofector® Kit
Catalog #: V4SP-5096
For medium-throughput transfection of primary cells, e.g. HUVEC, when working with the 4D-Nucleofector® 96-well Unit.
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P3 Primary Cell 4D-Nucleofector® LV Kit XL (5 x 1 RCT)
Catalog #: V4LP-3520
For larger scale transfection of primary cells, e.g. human T cells or CD34+ hematopoietic stem cells in the LV NucleocuvetteTM Cartridge format when working with the 4D-NucleofectorTM LV Unit
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SG Cell Line 4D-Nucleofector® X Kit L
Catalog #: V4XC-3012
For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-Nucleofector® X Unit (100 µL format).
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SG Cell Line 4D-Nucleofector® X Kit L
Catalog #: V4XC-3024
For efficient transfection of difficult-to-transfect cell lines, e.g. Ba/F3, Raji, or THP-1, in the 4D-Nucleofector® X Unit (100 µL format).
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SG Cell Line 96-well Nucleofector® Kit (960 RCT)
Catalog #: V4SC-3960
For medium-throughput transfection of cell lines, e.g. Ba/F3, Raji or THP-1, when working with the 4D-Nucleofector® 96-well Unit
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SG Cell Line 96-well Nucleofector® Kit
Catalog #: V4SC-3096
For medium-throughput transfection of cell lines, e.g. Ba/F3, Raji or THP-1, when working with the 4D-Nucleofector® 96-well Unit
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P4 Primary Cell 96-well Nucleofector® Kit (960 RCT)
Catalog #: V4SP-4960
For medium-throughput transfection of primary cells, e.g. mouse B-cells or immature DCs, when working with the 4D-Nucleofector® 96-well Unit.
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P4 Primary Cell 96-well Nucleofector® Kit
Catalog #: V4SP-4096
For medium-throughput transfection of primary cells, e.g. mouse B-cells or immature DCs, when working with the 4D-Nucleofector® 96-well Unit.
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Terms and ConditionsCD34+ cells BOGO
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive. -
CD34+ Cells for Cell and Gene Therapy Process DevelopmentIn this interview Dr. Manon Destalminil, Bioprocessing Project Leader at CellProthera, discusses the advantages of using research-grade CD34+ cells for cell and gene therapy process development. Please register to read.
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured. -
Human CD34+ Cells Technical information and instructions for use
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - HT Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell HT-Nucleofector™ Kit
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Transfection Protocol for Human CD34+ Cells - 4D-Nucleofector™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 4D-Nucleofector™ X Kit
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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Transfection Protocol for Human CD34+ Cells - Nucleofector™ I/II/2b Device SeriesInstructions of use for transfection of human CD34+ cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
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