ViaLight® Plus Cell Proliferation and Cytotoxicity BioAssay Kit, 500 Test
ViaLight® Plus Cell Proliferation and Cytotoxicity BioAssay Kit, 500 Test
ViaLight® Plus, 500 Tests
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Product Overview
ViaLight® Plus BioAssay Kits are designed to provide unprecedented speed and sensitivity for cytotoxicity and cell proliferation studies. The ViaLight® Assay incorporates bioluminescent detection of cellular ATP as a measure of viability. Measurement of ATP is the most accurate, effective, and direct way of determining the number of living cells in culture.
The ViaLight® Plus BioAssay is designed to deliver a high, stable luminescent signals for an extended period of time for greater experimental design flexibility. The easy two-step, non-radioactive assay is scalable for high-throughput applications in both 96-well and 384-well formats. The assay can be run on a variety of luminometers.
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Benefits
- Fast - Results from a 96 well plate can be processed and analyzed in as little as 10 minutes.
- Sensitive - Detect as few as 10 cells allowing for lower seeding densities and more assays
- Convenient - Simply add two reagents directly to your culture well and read. No cell washing or medium removal required.
- Flexible - Dynamic range of 5 decades and excellent performance with both adherent or suspension cultures allows you to design the experiment for your needs.
- Robust - Stable bioluminescent signal (half-life >5 hours) facilitates manual or batch processing. And, unlike MTT or Alamar Blue assays, ViaLight® Bioassays are not prone to interference due to culture condition changes.
Storage and Content
500 test kit (Sufficient for 5 plates)
- 1 x LT27-212 ATP monitoring reagent plus, Lyophilized
- 1 x LT27-079 Assay buffer, 50 mL bottle,
- 1 x LT27-076 Cell lysis reagent, 50 mL bottle
SDS, CoA, and Instructions
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Instructions - ViaLight™ Plus Kit
Instructions for proper use of the ViaLight™ Plus Kit -
Luminescent Cell-Based Assays
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Long-Term Culture of Primary Human Hepatocyte 3D Spheroids and Cytotoxicity Assay
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Determination of Spheroid Viability and Proliferation Using the ViaLight® Plus Cell Proliferation and Cytotoxicity BioAssay
Lonza's ViaLight® Plus Luminescent Cell Viability BioAssay can be used with spheroid 3D cell cultures with only slight modifications of the protocol. -
Stem Cell Research Tools for Biologically Relevant Results
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Measuring Hepatotoxicity in Primary Human Hepatocyte 3D Spheroid Using ViaLight Plus Cytotoxicity BioAssay
Protocol for using ViaLight Plus to measure cytotoxicity in hepatocyte 3D spheroids
Educational Material
Brochures, White Papers etc.
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Assessment of the Anti-angiogenic Effect of VEGFR2 siRNA in HUVEC
Scientific poster for AACR 2015 -
ViaLight® Plus Cell Proliferation Assay vs. MTT
Informational product application note -
Luminescent Cell-Based Assays
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Cell-based Assays Using Hard-to-transfect Cell Lines and Transiently Transfected Primary Cells
Co-sponsered poster with Promega -
Development of a Long-term Primary Hepatocyte 3D Spheroid Model
For Use in DILI Applications using ViaLight Plus Cytotoxicity BioAssay Kit
ViaLight® Plus Signal Stability Competitor Comparison
A Study of ViaLight® Plus Signal Stability Compared to a Leading Competitor
ViaLight® Plus Signal Stability Data
ViaLight® Plus BioAssay Kit Signal Stability Data
