Culture system containing MSCBM® Basal Media (PT-3238) and MSCGM® SingleQuots® Supplement Kit (PT-4105) required for proliferation of human bone marrow derived mesenchymal stem cells.
hMSC Chondrogenic Differentiation SingleQuots® Kit
X-VIVO® 15, with L-Glutamine, gentamicin, recombinant transferrin, and phenol red, xenofree, 1 L
X-VIVO® 15, Serum-free hematopoietic cell medium, with L-Glutamine, gentamicin and phenol red, xenofree, 500mL
Cryopreserved ampule of Human Peripheral Blood CD14+ Monocytes, negative selection, containing ≥10 million cells
Cryopreserved ampule of negatively selected Human Peripheral Blood CD14+ Monocytes, containing ≥ 50 million cells
TheraPEAK® MSCGMTM Mesenchymal Stem Cell Growth Medium BulletKit® including the basal medium and supplements, which is GMP-produced and serum-free, 1 L.
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Bioscience Solutions Catalog 2018Catalog-Chapter1-Stem-Cells-2018.pdf
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Hematopoietic and Immune Cell Products - Essential Tools for Hematopoietic ResearchLonza_Brochures_Hematopoietic_and_Immune_Cell_Products_-_Essential_Tools_for_Hematopoietic_Research.pdf
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Instructions and Technical Sheet- Human CD34+ Cells
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Instructions - Neural Progenitor Cell System, NHNPInstructions for Proper Use
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TechSheet - Neural Progenitor Cell System, NHNPTechnical Product Information Sheet
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References - X-Vivo™Overview of relevant references
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Live-cell imaging of CD34+ cells with Nanolive’s 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, in the presence of recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell migration was captured.
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Mitosis of CD34+ cells with Nanolive's 3D Cell ExplorerHuman cord blood CD34+ stem/progenitor cells were cultured in X-VIVO™ 15 serum-free hematopoietic cell medium, supplemented with recombinant human thrombopoietin (25ng/mL), Rlt3 ligand (25ng/mL) and stem cell factor (13ng/mL). The culture was coated with fibronectin. Live cell imaging was performed with Nanolive’s 3D Cell Explorer for 15 hours (3 images/min during 15hours) and cell division was captured. One can distinguish characteristic steps of mitosis affirming that Nanolive’s technology was completely non-invasive.
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Terms and ConditionsCD34+ cells BOGO
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Transfection Protocol for Human CD34+ Cells - 96-well Shuttle™ DeviceInstructions of use for transfection of human CD34+ cells with the P3 Primary Cell 96-well Nucleofector™ Kit