eCHO™ Basal Medium and Feed for Protein Production

Chinese hamster ovary (CHO) cells are the industry’s workhorse for therapeutic protein production. Most productions are in fed-batch or perfusion system using feeds to improve cell proliferation.

With over 40 years of expertise in media development, Lonza is proud to introduce eCHO^TM Basal Medium and Feed. eCHO^TM Medium is designed to maximize protein integrity, positively affected by low levels of lactate and late-stage cell viability. The serum-free, chemically defined, hydrolysate-free and non-animal origin (NAO) eCHO^TM Medium optimizes your cell proliferation and protein production.

Lonza has developed a new medium and feed for CHO cells with these benefits:

Easy, economical, chemically-defined, animal-origin-free medium
One part basal and one part feed formulation
Late-Stage Cell Viability and low lactate levels during production
High Protein Integrity
Consistent performance
Compatible with multiple cell clones
Scalability
GMP grade

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eCHO^TM Medium to Maximize Your Protein Production

Poster

Comparison data using eCHO^TM Basal Medium combined with eCHO^TM Feed in a fed-batch culture. Showing high cell viability and low lactate levels.

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Performance Features of the eCHO^TM Medium

Comparison of CHO Cell Proliferation and Protein Yield

Using eCHO^TM Basal Medium paired with eCHO^TM Feed results in a CHO medium showing high and consistent performance. Lonza was able to demonstrate consistent titer levels of ±3 g/L of recombinant protein with multiple CHO clones, multiple protein products, multiple lots, and multiple formats in 14-day fed-batch cultures. Titers were 2 - 3x higher compared to PowerCHO^TM 2.

The figure on the right shows offline monitoring of viable cell density (VCD) of fed-batch CHO cell cultures. Briefly, cells were cultured in automated ambr15 system with different media supplemented with different feeds as required. Cell densities were monitored using Vi-Cell (Beckman Coulter, USA) showing VCD (Y-axis) and process time (X-axis). With new Lonza eCHO^TM Media, the cell densities reached ~1.5 to 2-fold increase on Day 9 compared with other basal and feed media.

Right panel: showing the mAb production

Differences in Cell Viability of CHO Media compared to eCHO^TM Medium

eCHO^TM Basal Medium paired with eCHO^TM Feed was developed to have low cell death during the production process. Late stage cell viability results in low cell debris, having a positive effect on downstream processing, e.g. less filter clogging meaning less protein loss and faster purification.

The figure on the right shows offline monitoring of cell viability in shake flasks (working volume 30 ml) using Lonza eCHO^TM Media vs competitor media. The data points indicated were the average values of cell viability. Briefly, Lonza 30 represents 10% feed supplemented to the cultures on day 3, 5, and 7. Lonza 50 represents 10% feed supplemented to the cultures on day 3, 5, 7, 9, and 11. Lonza 30-6 represents 6% feed supplemented to the cultures on day 3, 5, 7, 9 and 11. All other competitors’ media and feed combinations were denoted as A1-3, B1-3, C1, D1-3, E1-2, F1-2, G1, P1-2 and V1-3 respectively.

Lactate concentration of CHO Media compared to eCHO^TM Medium

During protein production in eCHO^TM Medium low lactate levels are a key contributor to protein integrity and improved post translational modifications. eCHO^TM Basal Medium and eCHO^TM Feed show consistent low levels of lactate during protein production.

The figure on the right shows the Lactate concentration profile in shake flasks using different combinations of basal and feed media using Lonza eCHO^TM Media vs competitor media. Briefly, Lonza 30 represents 10% feed supplemented to the cultures on day 3, 5, and 7. Lonza 50 represents 10% feed supple-mented to the cultures on day 3, 5, 7, 9, and 11. Lonza 30-6 represents 6% feed supplemented to the cultures on day 3, 5, 7, 9 and 11. All other competitor’s media and feed combinations were denoted as 1–30 and 38–50 respectively.

Paired medium and feed

Associated basal medium and feed, ready to use

Composition

Chemically defined, non-animal origin

Documents

Certificate of analysis, MSDS, manual

Storage

2 - 8 °C, protect from light

Manufactured

GMP, labelled “For Further Manufacturing”

Format

1L bottle liquid, powder >10L

Shelf life

Liquid minimum >6 months (shelf life study),
Powder minimum 2 years

BEBP12-933Q

eCHO™ Basal Medium

1L

Liquid

BE15-933D

eCHO™ Basal Medium

10L

Powder

BEBP12-932Q

eCHO™ Feed

1L

Liquid

BE15-932D

eCHO™ Feed

10L

Powder

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Maximize your protein production with eCHO™ Basal Medium and eCHO™ Feed

Video

An introduction to Lonza’s recently launched medium and feed for the production of recombinant proteins, monoclonal antibodies and biosimilars in CHO cells.

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eCHO^TM Medium to Maximize Your Protein Production

Poster

Comparison data using eCHO^TM Basal Medium combined with eCHO^TM Feed in a fed-batch culture. Showing high cell viability and low lactate levels.

Download poster

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eCHO™ Medium for Protein Production

eCHO™ Medium for Protein Production

eCHO™ Basal Medium and Feed for CHO Cell Protein Production

eCHOTM Medium to Maximize Your Protein Production

Poster

Performance Features of the eCHOTM Medium

Comparison of CHO Cell Proliferation and Protein Yield

Differences in Cell Viability of CHO Media compared to eCHOTM Medium

Lactate concentration of CHO Media compared to eCHOTM Medium

Maximize your protein production with eCHO™ Basal Medium and eCHO™ Feed

Video

eCHOTM Medium to Maximize Your Protein Production

Poster

Interested in our Webinars?

eCHO^TM Medium to Maximize Your Protein Production

Performance Features of the eCHO^TM Medium

Differences in Cell Viability of CHO Media compared to eCHO^TM Medium

Lactate concentration of CHO Media compared to eCHO^TM Medium

eCHO^TM Medium to Maximize Your Protein Production