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  • Instructions -ACK Lysing Buffer
    How to use ACK lysis buffer
  • TechSheet – Rat Calvariae Osteoblast Cell System (R-Ost-583)
    Technical tips for culturing Rat Calvariae Osteoblast Cells
  • Two Distinct Activation Methods Yield Clinical-Scale Expansion of Peripheral Blood Derived γδ T Cells
    In this study, we demonstrate that billions of γδ T cells can be generated from peripheral blood mononuclear cells (PBMCs) with two distinct activation methods, using TheraPEAK® T-VIVO® Medium.
  • Non-viral Gene Modification for Ex-vivo Cell Therapy
    In this interview our Director Transfection R&D discusses the advantages of non-viral transfection technologies for CRISPR- or transposon-based genetic modification cell and gene therapy.
  • A Novel, Chemically Defined, Serum-Free Cell Culture Medium for Expansion of Transfected T Cells
    In this study, we present data generated with TheraPEAK® T-VIVO®, a novel, chemically defined, non-animal origin cell culture medium utilized to grow T cells in the absence of serum, before and after transfection with the Nucleofector® Technology.
  • A Serum-free Chemically Defined Medium for the Expansion of NHDF-Adult
    Traditionally, in vitro cell culture medium contains animal-derived products like serum, tissue extracts or hydrosylates. They are also a source of undesirable factors, such as endotoxin, and pose potential BSE risks. The undefined and variable nature of these components is a challenge in basic research and applied biomedical sciences. Lonza has developed a serum-free chemically defined, fibroblast growth medium, to propagate primary adult normal human dermal fibroblasts (NHDF).
  • Transfection Protocol for Human MSC - Nucleofector™ I/II/2b Device Series
    Instructions of use for transfection of human mesenchymal stem cells with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
  • Using Serum-free Media to Streamline CAR T-Cell Manufacturing Workflows
    Learn from this peer reviewed article, published in Cell and Gene Therapy Insights, how serum-free media can support large-scale, regulatory compliant expansion of CAR T-Cells.
  • Transfection Protocol for Human MSC - 4D-Nucleofector™ Device
    Instructions of use for transfection of human mesenchymal stem cells with the P1 Primary Cell 4D-Nucleofector™ X Kit
  • TechSheet - X-VIVO™ Media Systems
    Instructions for how to use the X-VIVO™ Media Series