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Pharma & Biotech

Basic NucleofectorTM Kit for Primary Mammalian Endothelial Cells

Catalog #: VAPI-1001
For transfection of mammalian endothelial cells lacking a cell type specific Optimized Protocol for the NucleofectorTM I/II/2b Device
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Product Overview

In addition to various cell type-specific kits for primary endothelial cells, we also provide a cell group-specific Basic NucleofectorTM Kit. This kit is suited to determine the optimal transfection conditions for any mammalian endothelial cell type that lacks a cell type specific Optimized Protocol for the NucleofectorTM 2b Device (or older generations I or II). In a first experiment a selection of different NucleofectorTM Programs is tested in combination with the Basic NucleofectorTM Solution for endothelial cells. The kit is available in different variants for either 10 or 25 or 4 x 25 reactions.

Benefits

  • Optimizations can be performed within 1 experiment 
  • Detailed protocol guiding through the optimization procedure 
  • Optional result fine tuning with help of our Scientific Support Team 
  • Transfection efficiencies up to 90%
  • Viabilities up to 85%

Applications

  • Kit suited for endothelial cells from different mammalian species and various organs 
  • Already tested for human pulmonary artery endothelial cells

Content & Storage

Content

2 x 0.45 mL NucleofectorTM Solution for Primary Mammalian Endothelial Cells
2 x 0.1 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
10 Aluminum cuvette (100 µL)
10 Single use pipettes

Instructions

Safety Data Sheets (SDS)

Certificate of Analysis (CoA)

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