Cell Line Nucleofector® Kit T
Cell Line Nucleofector® Kit T
For efficient transfection of cell lines, e.g. A549, Caco-2, CHO-K1, HT-, or U-87 MG, in the Nucleofector® I/II/2b Device
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Product Overview
The Cell Line Nucleofector® Kit T is one of our five Nucleofector® Kits suited for transfecting cell lines in the Nucleofector® 2b System. This kit has been determined to be optimal for transfecting following cell types:
A431
A549
Caco-2
CHO-K1
D1 ORL UVA
HT-1080
NALM-6 (DSMZ)
NRK
P815
RBL-2H3
T84
TF-1
U-87 MG
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.
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Benefits
- High transfection efficiencies in difficult-to-transfect cell lines
Applications
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
- Low-throughput transfection in single cuvette format
Storage and Content
2.25 mL Nucleofector® Solution T
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
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Certificate of Analysis (CoA)
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