Mouse Dendritic Cell Nucleofector™ Kit
Mouse Dendritic Cell Nucleofector™ Kit
Product Overview
The NucleofectorTM 2b Device (or older generations I or II) works cell type specific kits, each of them dedicated to an individual primary cell. This NucleofectorTM Kit is the optimal kit for efficient transfectiion of primary mouse dendritic cells (immature or mature).
The respective cell type-specific Optimized Protocol or recommendation is available under "Instructions" or in our knowledge database. In this Optimized Protocol the best Nucleofection conditions are indicated. In addition, we share our experience and knowledge for treatment of this cell type.
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Benefits
- High transfection efficiencies for primary cells
- Cells maintain functionality post transfection
Applications
- Transfection of cell numbers in the range of 2 × 105 to 2 × 107 cells
- Low-throughput transfection in single cuvette format
Storage and Content
2.25 mL NucleofectorTM Solution for Mouse Dendritic Cells
0.5 mL Supplement 1
30 µg pmaxGFPTM Vector (0.5 μg/μl in 10 mM Tris pH 8.0)
25 Aluminum cuvette (100 µL)
25 Single use pipettes
SDS, CoA, and Instructions
Safety Data Sheets (SDS)
Choose a language to view the SDS.
Certificate of Analysis (CoA)
Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.
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Transfection Protocol for Mature mDC (BALB/c) - Nucleofector™ I/II/2b Device Series
Instructions of use for transfection of mature mouse dendritic cells (BALB/c) with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S) -
Transfection Protocol for Mature mDC (C57BL/6) - Nucleofector™ I/II/2b Device Series
Instructions of use for transfection of mature mouse dendritic cells (C57BL/6) with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S) -
Transfection Protocol for Immature mDC (C57BL/6) - Nucleofector™ I/II/2b Device
Instructions of use for transfection of immature mouse dendritic cells (C57BL/6) with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S) -
Transfection Protocol for Immature mDC (BALB/c) - Nucleofector™ I/II/2b Device
Instructions of use for transfection of immature mouse dendritic cells (BALB/c) with Nucleofector™ 1/2 Devices (incl. 1, 2b, 2N, 2S)
